摘要
目的:探讨纳米银(silvernanoparticles,nanosilver,Nano-Ag)对季节性甲型流感病毒H1N1的抑制作用。方法:应用血球凝集试验、鸡胚培养法和细胞病变(cytopathiceffect,CPE)观察,检测Nano-Ag对季节性流感病毒H1N1的抑制作用。结果:Nano-Ag/H1N1组和H1N1对照组血球凝集试验的效价分别为1:4和1:1024,两组相比有统计学显著差异(P<0.001);Nano-Ag可有效抑制H1N1在鸡胚中的增殖,尿囊液血凝试验为阴性,与H1N1对照组(血凝效价1:1024)相比有显著差异(P<0.001);25μg.mL-1Nano-Ag可有效抑制H1N1细胞病变效应和细胞凋亡,细胞凋亡率由(22.0±2.4)%降至(7.3±4.0)%(P<0.001)。结论:Nano-Ag对季节性流感病毒H1N1具有明显的抑制作用。
Objective：To explore the inhibiting effect of silver nanoparticles （nanosilver,Nano-Ag） on influenza virus H1N1.Methods：By hemagglutination test,chicken embryos cultivation and cytopathic effect （CPE）,the effect of Nano-Ag on influenza virus H1N1 was detected.Results：The hemagglutination titer was 1：4 in Nano-Ag/H1N1 group and 1：1 024 in the control group of influenza viruses H1N1（P〈0.001）.Nano-Ag effectively inhibited influenza virus H1N1 proliferation in of chicken embryos,and the hemagglutination was negative in allantoic fluids of Nano-Ag/H1N1 group.However,the hemagglutination titer was 1：1 024（P〈0.001） in the control group.Nano-Ag 25 μg·mL^-1 inhibited cytopathic effect and cell apoptosis induced by the influenza virus H1N1;apoptosis rate reduced from （22.0±2.4）% in the control group to （7.3±4.0）% in Nano-Ag/H1N1 group（P〈0.001）.Conclusion：Silver nanoparticles display obvious inhibiting effect on influenza virus H1N1.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2010年第7期605-611,共7页
Chinese Journal of New Drugs
基金
国家自然科学基金(30870681)
辽宁省自然科学基金(20062133)
