二步免疫印迹法的建立及在诊断类风湿关节炎中的意义

Establishment of two-step immunoblot assay and its clinical significance in the diagnosis of rheumatoid arthritis

建立类风湿关节炎(RA)的特异性诊断方法。方法 用制得的可溶性核提取物为抗原,用免疫印迹法检测类风湿疾病患者血清中的RA33抗体。再以ENA为抗原,第2次用免疫印迹法检测RA33抗体阳性的病人血清中的RNP和Sm抗体。结果和结论 60例RA病人中22例阳性(36.6％),60例系统性红斑狼疮(SLE)病人中12例阳性(20％),两病的RA33抗体阳性率有显著性差别(P<0.05),而122例其他风湿性疾病和正常对照血清全为阴性。第2次免疫印迹法检测发现83％的RA33抗体阳性的SLE病人抗RNP(75％)和(或)抗Sm(33％)抗体为阳性,RA病人全阴性。这样用二步免疫印迹法检测可相对特异地诊断RA。

To establish specific detecting method of RA. Methods Anti-RA33 antibody in rheumatoid diseases was detected by immunobolt assay with soluble nuclear antigens .The RNP and Sm antibody in RA33 antibody positive sera was detected by immunobolt assay again with ENA antigen. Results and conclusion We detected RA33 antibody in 22 of 60 sera(36.6%)from RA patients and in 12 of 60 sera from SLE patients (20%). There was a significant difference of positive rate between the two(P<0.05).And there was no positive reaction in other 82 connective tissue diseases and 40 normal controls ,In the second-step immunoblot assay.it was found that anti-RNP antibody and /or anti-Sm antibody in RA patients were all negative and 83 percent of SLE were positive.So the method of two梥tep immunoblot assay is of signififcance in diagnosis of RA.