HiHiLo实施过程中网织红细胞与Hb变化规律的研究

The Relationships between Reticulocytes and Hb Variation during HiHiLo

目的:观察HiHiLo实施过程中受试者网织红细胞的变化规律,探寻网织红细胞与Hb变化的关系。方法:以77名竞技体育学院和体育教育学院在校男性大学生为研究对象,实施为期30天的HiHiLo计划。受试者每晚在氧浓度为14.8%～14.3%(模拟海拔约2800～3000m)的常压低氧舱中暴露至少10h,每周进行3次低氧环境下运动,运动方式为蹬功率自行车,每次30min,运动强度以个体75%V.O2max强度为基础,运动过程中通过调节氧气浓度和/或功率自行车负荷保持SpO2<92%,功率自行车转数为60rpm,受试者的日常训练在常氧环境下进行。在HiHiLo前(第1天)、HiHiLo的第4天、第7天、第16天、第23天和第30天测试受试者的血象指标,包括Hb、RBC、Hct和网织红细胞相关参数。结果:30天的HiHiLo低氧训练计划,可显著增加受试者的网织红细胞和Hb。网织红细胞在第7天时极其显著(P<0.01)升高,Hb、RBC、Hct在第16天时极显著(P<0.01)升高。结论:HiHiLo可有效促进红细胞生成,增加受试者的RBC和Hb,提高机体氧运输能力,且网织红细胞的显著性变化早于RBC、Hb的显著性变化。

Objective：The present study analyzed the disciplinarians of reticulocytes and hameglobin during HiHiLo and explored the correlation between reticulocytes and effectiveness of HiHiLo.Methods：The subjects were 77 male students from the department of physical education in Beijing Sports University.The subjects were exposed in hypoxic enviorment（O2%：14.8%~14.3% equivalent to the altitude of 2 800~3 000m）for more than 10 hours every night lasting 30 days.Besides participating the routine training at sea level,the subjects completed 3 training sections（bicycle ergometer exercise at the intensity of 75% O2max with the cadence of 60 rmp/min,lasting for 30min）in hypoxia every week.During the hypoxic erercise,the SpO2 was controlled less than 92%,through adjusting the oxygen concentration and/or the exercise load before HiHiLo,during HiHiLo,at day4,day7,day16,day23 and day30,the blood samples for the indexes of reticulocytes and Hb,et al.were obtained.Results：During the 1 month period of HiHiLo,reticulocytes and Hb were significantly increased.At day 7,reticulocytes were significantly increased,but Hb,RBC and Hct were significantly increased at day 16.Conclusion：HiHiLo can promote the production of RBC,Hb effectively,improve the capacity of oxygen transportation.The significant change of reticulocyte is earlier than the significant changes of RBC and Hb.