间歇运动训练和急性间歇运动对缺血/再灌注大鼠心电图及血清心肌酶的影响

The Effects of Interval Training and Acute Interval Exercise on Ischemia-reperfusion rats' ECG and Serum Myocardial Enzymes

目的:探索长期运动训练和急性运动的心脏保护作用及心电指标的变化规律,为进一步研究运动预适应机制提供依据。方法:32只3月龄雌性SD大鼠随机分成3大组:间训模型组(n=8)、急性运动模型组和对照组(n=8),间歇运动训练组进行高强度间歇运动训练,急性间歇运动组仅进行急性高强度的间歇运动,对照组(n=16)不运动,在心脏模型制备时对照组随机分成对照模型组(n=8)和对照假手术组(n=8)。运动训练结束后,各组大鼠均采用结扎左冠状动脉制备在体大鼠心肌缺血/再灌注损伤模型,但对照假手术组只开胸穿线而不结扎。缺血/再灌注过程实行全程心电监控,缺血30min、再灌注40min后,测定血清心肌酶。结果:经间歇运动训练和急性间歇运动处理的缺血/再灌注大鼠血清心肌酶明显低于对照模型组(P<0.05或P<0.01);缺血30min及再灌注40min间歇运动训练组心电图ST段、T波面积和QT间期明显低于对照模型组(P<0.05或P<0.01);再灌注40min急性间歇运动组心电图ST段、T波和QT间期明显低于对照模型组(P<0.05)。结论:长期间歇运动训练产生心肌抗缺血/再灌注损伤的保护作用,急性运动主要产生抗心脏再灌注损伤的保护作用。

Objective：Exploring the cardio-protection and the variation of ECG if I/R rats in long-term sports training and acute exercise,and provide basis for further research on mechanism of exercise preconditioning.Methods：Thirty-two SD rats were randomly divided into four groups：interval exercise training group（IET,n=8）,acute interval exercise group（AIE,n=8）,the control group（C,n=8）and sham operated group（S,n=8）.But sham operated group,the other groups were carried out ischemia reperfusion experiment.Myocardial ischemia reperfusion injure was induced by the occlusion of left anterior descending branch of the coronary artery.ECG was monitored during whole I/R.After thirty minutes ischemia and forty minutes reperfusion,activity of serum myocardial enzymes was observed.Results：The activity of serum myocardial enzymes in IET and AIE were remarkably lower than the control group（P0.05 or P0.01）;ST segment,T wave area and Q-T interval in IET were remarkably lower than the control group at 30 min ischemia and 40 min reperfusion（P0.05 or P0.01）;ST segment,T wave area and Q-T interval in AIE were remarkably lower than the control group at 40 min reperfusion（P0.05）.Conclusion：Long-term exercise can induce heart protective effect against injury induced by ischemia and reperfusion.Acute exercise can induce heart protective effect against injury induced by reperfusion.