牛磺胆酸钠诱导急性坏死性胰腺炎大鼠模型的理想浓度

Optimal concentration of sodium taurocholate inducing acute necrosis pancreatitis in a rat

目的 探讨牛磺胆酸钠诱导急性坏死性胰腺炎（ANP）并发多器官功能衰竭（MODS）大鼠模型的量-效关系及理想浓度.方法 280只大鼠按数字随机法分为对照组（40只）和ANP组,分别采用经胰胆管逆行注射3.5%、4%、4.5%、5%牛磺胆酸钠制备ANP模型（每浓度组60只）.术后1、3、6、12、24 h分批处死大鼠,检测血清淀粉酶、总胆红素、肌酐水平,行血气分析,常规胰腺病理检查并评分;各ANP组另留20只大鼠观察建模后24 h生存率.结果 各ANP组的血清淀粉酶、总胆红素、肌酐水平及氧合指数（PaO2/FiO2）、胰腺病理评分均随牛磺胆酸钠浓度增加而升高,呈明显的量-效和时-效关系.4.5%牛磺胆酸钠建模组于术后6 h出现肝、肾功能障碍高峰,12 h出现呼吸功能障碍高峰,其MODS发生率为52.5%,显著高于3.5%、4.0%牛磺胆酸钠建模组的15.0%和37.5%;24 h生存率为65%,显著高于5%牛磺胆酸钠建模组的30%.结论 4.5%牛磺胆酸钠诱导ANP大鼠模型具有较高的MODS发生率和24 h生存率,是ANP并发MODS诊治研究的理想建模浓度.

Objective To explore the dose-effect relationship and optimal concentration of sodium taurocholate （NaTc） to establish acute necrosis pancreatitis （ANP） complicating MODS in a rat. Methods 280 SD rats were randomly divided into control group （n =40） and 3.5%, 4%, 4.5%, 5% ANP group （n = 60, respectively）. ANP groups were induced by retrograde injection of NaTc solution into the pancreatic duct of rats. Rats in each model group were sacrificed at 1, 3, 6, 12 and 24 h after the model induction. Serum amylase, serum total bilirubin, creatinine, blood gas analysis, and pathological changes of pancreas were determined. Survival rate at 24 h after the model induction was observed in other 20 ANP rats in each group. Results In each groups, the serum amylase level, creatinine, blood gas analysis and pathological changes all increased corresponding to the increase of NaTc, and there was significant dose-effect relationship and time-effect. In 4.5% group, the dysfunction of liver and kidney reached the peak at 6 h,while the respiratory dysfunction reached its peak at 12 h. Its incidence of MODS was 52.5%, which was significantly higher than those in 3.5%, 4.0% group （15.0% and 37.5%） ;and 24 h survival rate of 4.5% group was 65%, which was significantly higher than that in 5% group （30%）. Conclusions 4.5% NaTc solution may be the optimal concentration to establish the ANP model, which had higher incidence of MODS and 24 h survival rate.