树突状细胞在多发性硬化免疫机制和治疗中作用的研究

Study on roles of dendritic cell in immunologic mechanism and therapy of multiple sclerosis

目的研究树突状细胞(dendritic cell,DCs)在多发性硬化(multiple sclerosis,MS)免疫机制及治疗中的作用。方法建立实验性变态反应性脑脊髓膜炎(EAE)E组、对照(C)组和耐受组模型,采用混和淋巴细胞反应(MLR)检测各组脾脏DC培养上清液刺激淋巴细胞增殖的能力,采用ELISA双夹心法测量各组脾脏DC培养液中细胞因子水平。结果三组脾DC刺激淋巴细胞增殖能力的差异具有统计学意义,EAE组最强、EAE耐受组最弱(P<0.05,P<0.01,P<0.01)。三组模型脾脏DC培养上清液的TNF-α、IL-10及IL-12水平每两组间进行比较,除了EAE组和对照组及EAE与耐受组之间比较IL-10水平没有统计学差异外(P>0.05),余均有统计学差异(分别为P<0.01,P<0.05)。结论DC具有功能异质性和功能可调性,DC调控免疫反应类型与其调节淋巴细胞的增殖和其分泌细胞因子水平有密切关系,可能在MS免疫机制及治疗中起重要作用。

Objective To study the effect of dendritic cell（DC） in the immunologic mechanism and therapy of multiple sclerosis.Methods Three groups including the model of experimental allergic encephalomyelitis（EAE）,control and immunotolerance were established,Mixed leukocyte reaction was performed to detect the ability of culture solution of splenic DC to stimulate lymphocytic proliferation.Cytokines produced by splenic DC of each group was measured by ELISA.Results There were differences among the abilities of splenic DC of three groups to stimulate lymphocytic proliferation.The ability of EAE group was the strongest,and of EAE immunotolerance group was the most weak（P〈0.05,P〈0.01,P〈0.01）.The level of cytokines（TNF-α,IL-10 and IL-12） produced by splenic DC were compared between each two groups,the differences were remarkbly visible（P〈0.01,P〈0.05） except the difference of IL-10 between EAE group and control group and between EAE group and immunotolerance group（P〉0.05）.Conclusion DC can possess the heterogeneity and adjustability on function.DC can control the type of immune responses through modulating the lymphocytic proliferation and the production of cytokines.DC may play an important role in immunologic mechanism and therapy of multiple sclerosis.