家族遗传与非家族遗传性病理性瘢痕的基因芯片谱研究

Analysis of differential gene expression between heredofamilial and no-heredofamilial pathological scars

目的应用cDNA微阵列(基因芯片)技术和生物信息学分析对家族遗传与非遗传性病理性瘢痕基因表达差异进行分析和研究。方法采集临床的遗传性与非遗传性病理性瘢痕样品各5例,将480种人类基因PCR产物制成微阵列表达谱芯片;提取瘢痕疙瘩和正常皮肤的总RNA,并纯化mRNA。等量的两种瘢痕组织的mRNA分别进行标记,合成,杂交,洗膜后,计算机扫描分析比较两种组织中差异表达的基因,并利用生物学数据库进行分析。结果遗传性瘢痕组织与非遗传性瘢痕组织相比,显著差异表达基因有65个,最后通过生物信息学分析确定29个差异表达基因,构成遗传性瘢痕与非遗传性瘢痕的差异表达谱。结论遗传性瘢痕的形成是复杂的病理生理过程,受多基因表达调控。进一步的研究将有助于揭示遗传性瘢痕形成机制以及针对遗传性瘢痕的治疗。

Objective To investigate and analyse the differential gene expression between heredofamilial and no-heredofamilial pathological scars by using cDNA microarray and bioinformatics analysis.Methods Five clinical pathological samples of heredofamilial and no-heredofamilial scars were collected;cDNA microarray was made of PCR products of 480 human genes;the total RNA of keloids and normal skin were extracted and purified into mRNA.After the equal amounts of mRNA of two kinds of scar tissue were marked,integrated,hybridized and washed,the discrepant expression genes were sacnned and comprised by computer and analyzed by biological database.Results Sixty-five genes of significantly discrepant expression were discovered by compared with the two kinds of scars.Then,the final 29 genes were identified by bioinformatic analysis,which were constituted differential expression profiling of heredofamilial and no-heredofamilial scars.Conclusion A variety of genes regulates the formation of heredofamilial pathological scars,which is a complicated pathophysiology process.Further analysis of the identified genes may be useful for revealing the molecular mechanism of heredofamilial pathological scars and helpful for the therapy of heredofamilial pathological scars.