子宫内膜异位症患者在位内膜中cofilin-1蛋白表达水平与其种植能力的关系

Relationship between cofilin-1 expression and implantation capacity in eutopic endometrium of patient with endometriosis

目的 探讨子宫内膜异位症（内异症）患者在位内膜中cofilin-1（CFL1）蛋白表达水平与其种植能力的关系.方法 选择2008年3-12月在中国医科大学附属盛京医院妇产科经腹腔镜或开腹手术证实并接受子宫全切除术的20例Ⅲ～Ⅳ期内异症患者为内异症组,同期经病理检查证实为宫颈原位癌并接受子宫全切除术的患者20例为对照组.收集两组患者的子宫内膜组织,采用腹腔注射法分别建立内异症裸鼠模型,计算各组的建模成功率及裸鼠体内异位病灶的体积;采用蛋白免疫印迹法检测两组子宫内膜组织及裸鼠体内异位病灶中CFL1蛋白的表达水平;采用免疫组化染色法检测两组子宫内膜组织中CFL1蛋白阳性表达率.结果 内异症组裸鼠模型的异位病灶平均体积为（2.38±0.22）mm^3,对照组为（0.36±0.08）mm^3,两组比较,差异有统计学意义（P〈0.05）;内异症组建模成功率为95%（19/20）,对照组为5%（1/20）,两组比较,差异也有统计学意义（P〈0.05）.内异症组子宫内膜中CFL1蛋白表达水平、阳性表达率及裸鼠模型异位病灶中CFL1蛋白表达水平分别为0.82 ±0.06、90%（18/20）、0.85±0.03,对照组分别为0.21±0.03、20%（4/20）、0.22±0.02,两组分别比较,差异均有统计学意义（P〈0.05）.两组40例患者的子宫内膜组织中,CFL1蛋白表达阳性及阴性者的建模成功率分别为86%（19/22）和6%（1/18）,两者比较,差异有统计学意义（P〈0.05）.结论 内异症患者在位内膜体内种植能力及CFL1表达水平高于正常内膜,CFL1高表达可能与子宫内膜异位症在位内膜较强的种植能力密切相关.

Objective To investigate the relationship between expression of eofilin-1 and in vivo implantation capacity of eutopic endometrium of endometriosis.Methods Eutopic endometrium of 20 cases with stage Ⅲ or Ⅳ endometriosis were obtained by laparoscopic or laparotomy surgery （endometriosis group） matched with 20 cases of eutopic endometrium from patients with cervical cancer in situ （control group） in Department of Obstetrics and Gynecology,ShengJing Hospital.All cases＇ eutopic endometrium were collected and injected into abdominal cavity of nude mice to establish endometriosis animal model, then the successful rate of animal model and volume of endometriosis lesion were calculated.The expression and positive rate of eofilin-1 protein were measured by western blot and immunohistochemistry staining.Results The mean volume of endometriosis lesions （2.38 ± 0.22）mm^3 in endometriosis group was significantly bigger than （0.36 ± 0.08） mm^3 in control group （P 〈 0.05）.The successful rate of establishing endometriosis model was 95% （19/20） in endometriosis group and 5% （1/20）in control group, which reached statistical difference（P 〈0.05）.The expression and positive rate of cofilin-1 protein in eutopic endometrium and the expression of cofilin-1 protein in endometriosis lesion of animal model were 0.82±0.06, 90% （18/20）, 0.85 ± 0.03 and 0.21 ± 0.03, 20% （4/20）, 0.22 ± 0.02 in control group, which reached statistical difference（P 〈0.05）.The successful rate of establishing endometriosis model with positive cofilin-1 in endometrium 86% （19/22） was significantly higher than 6% （1/16） of model＇s endometrium with negative cofilin-1 expression（P 〈0.05）.Conclusions Implanting capacity and cofilin-1 expression level of eutopic endometrium of endometriosis were more intensive than that of normal endometrium.High cofilin-1 expression was probably related with implanting capacity of eutopic endometrium of endometriosis.