改良胰腺灌注方法对大鼠供体胰腺质量影响的实验研究

The Experimental Study of Effects of Improved Method for Pancreas Perfusion on Donor Pancreas in Rats

目的 通过建立大鼠胰腺的灌注模型,探讨胰腺移植供体胰灌注过程中开放门静脉为灌注液流出道对供体胰腺的保护作用.方法 48只雄性Wister大鼠随机分为实验组、对照组,供体胰腺灌注过程中,实验组开放门静脉为灌注液流出道,对照组开放肝后腔静脉（右心房）为灌注液流出道.两组均用UW液作为灌注保存液,分别于灌注冷保存后6,10,14h（n=24）,切取胰腺标本制成石蜡切片,经HE染色进行组织病理学观察,P-选择素（P-selection,PS）、细胞间黏附分子-1（ICAM-1）免疫组化染色以及制作电镜标本进行细胞超微结构的观察.结果 对照组胰腺组织病理损伤、免疫组化以及电镜下细胞超微结构的改变均较明显,且随着时间的延长组织损伤愈发严重.实验组胰腺组织损伤较轻,两组比较差异有统计学意义（P〈0.05）.结论 胰腺移植供体胰腺灌注过程中,早期开放门静脉相对于开放肝后腔静脉为灌注液流出道,能够减轻灌注损伤,对供体胰腺起保护作用.

Objective To establish the model of pancreas pertusion in Wiser rats, to explore the protective effect of perfusion with portal vein as the drainage on the donor pancreas. Methods Forty-eight Wister rats were randomized divided into the experiment group and control group. In the process of donor pancreas perfusion,the rats in experiment group recieved perfusion with portal vein as the drainage while the control group with the inferior caval vein （ right atrium）. Both the two groups were perfused by UW solution. After perfusion in cold storage 6,10,14hours（ n = 24） ,the pancreas specimen was cut into paraffin slices. P-selection（PS） and intercellular Adhesion Molecules-I （ICAM- 1 } were detected using immunohistochemical staining and the ultrastructure was observed by electron microscope. Results The optical and electron microscope showed that the damage of pancreas graft tissue was obvious and aggravated grad- ually as perfusion tieme prolonged in the control group. The immunohistochmistry staining showed that the expression of PS and ICAM-I were augmented gradually as the injury aggravated,while these changes were mild in the experimental group,and the difference was statistically significant （ P 〈 0.05 ）. Conclusion In the process of pancreatic perfusion, perfusing through portal vein as the draiage caused less damage compared with the inferior caval vein as the drainage, the former can lessen the injury to the pancreas.