门静脉高压症脾亢患者外周血CD4^(+)CD25^(+)CD127^(low/-)调节性T细胞含量与免疫功能研究

Research on the content and the immune function of CD4^(+)CD25^(+)CD127^(low/-) regulatory T cells in peripheral blood of patients with portal hypertension and hypersplenism

目的研究门静脉高压症(PHT)脾亢患者外周血CD4+CD25+CD127low/-调节性T细胞(Treg)与CD3+、CD4+、CD8+T细胞的表达变化,探讨脾脏免疫功能改变及其可能的调节机制。方法PHT脾亢患者20例(脾亢组),健康成人18例(对照组),采用流式细胞术检测外周血中CD4+CD25+CD127low/-Treg及CD3+、CD4+、CD8+T细胞的表达变化。结果脾亢组外周血中CD4+CD25+CD127low/-Treg占CD4+T细胞的比例为(5.3±3.0)%,明显高于对照组的(2.5±0.9)%(P<0.01)。脾亢组CD3+、CD4+、CD8+T细胞含量均低于对照组(P<0.05)。CD4+CD25+CD127low/-Treg的含量与CD3+T细胞比例呈负相关(r=-0.630,P<0.01),与CD4+T细胞比例呈负相关(r=-0.561,P<0.05)。结论PHT脾功能亢进患者CD4+CD25+CD127low/-调节性T细胞表达量增多,可能对脾脏免疫功能的调节有重要作用。

Objective To investigate the changes of expression of CD4＋CD25＋CD127low/-regulatory T cells and CD3＋,CD4＋,CD8＋ T cell in peripheral blood of patients with portal hypertension（PHT） and hyper-splenism,explore the changes in splenic immune function and its possible regulative mechanism.Methods The ratios of CD4＋CD25＋CD127low/-Treg and CD3＋,CD4＋,CD8＋ T cell in peripheral venous blood were exam-ined with flow cytometry in 20 patients with PHT and hypersplenism,and compared with 18 healthy individuals.Results The ratios of CD4＋CD25＋CD127low/-/CD4＋ in PHT group were（5.3±3.0）%.It was significantly in-creased compared with healthy group（2.5±0.9）%（P0.01）.The ratios of CD3＋,CD4＋,CD8＋ T cells were decreased in patients with PHT compared with healthy group（P0.05）.There was a negative correlation between CD4＋CD25＋CD127low/-and CD3＋,CD4＋（r=-0.630,P0.01;r=-0.561,P0.05）.Conclusion The expression of CD4＋CD25＋CD127low/-Treg are significantly increassed in patients with PHT and hypersplenism,it may play an important role in splenic immunomodulatory.