肝癌患者射频消融后血清Th1、Th2型细胞因子的变化

Changes of Th1,Th2 cytokines in the serum of patients with hepatocellular carcinoma after radiofrequency ablation

目的研究肝癌患者射频消融(RFA)治疗后血清Th1、Th2类细胞因子水平的变化,评价射频消融治疗对肝癌患者免疫状态的影响。方法多极射频消融治疗仪(RFA-I)治疗肝癌患者28例,采用流式细胞术检测患者在射频消融前和治疗后1d、21d外周血Th1细胞因子(IL-2、TNF-α)及Th2细胞因子(IL-4、IL-10)。结果肝癌患者治疗前外周血Th1细胞因子(IL-2、TNF-α)降低,Th2细胞因子(IL-4、IL-10)升高,与对照组相比,差异有统计学意义(P<0.05);RFA治疗后外周血Th1细胞因子(IL-2、TNF-α)升高,Th2细胞因子(IL-4、IL-10)降低,与治疗前相比,差异有统计学意义(P<0.05)。结论射频消融治疗可改善肝癌患者机体的免疫功能。

Objective To study the changes of Th1,Th2 type cytokines in the serum of patients with hepatocellular carcinoma（HHC） after radiofrequency ablation（RFA）.To evaluate the influence of RFA on immunity function in patients with HHC.Methods Twenty-eight cases of HHC patients were treated using RFA-I radio frequency instrument.Th1 type cytokines（IL-2,TNF-α） and Th2 type cytokines（IL-4,IL-10） in the serum were detected by flow cytometry.Results The level of Th1 type cytokines（IL-2,TNF-α） was significantly lower and Th2 type cytokines（IL-4,IL-10） higher than those of the control group,respectively（P0.05）.After treatment with RFA,the level of Th1 type cytokines（IL-2,TNF-α） increased while the level of Th2 type cytokines（IL-4,IL-10） decreased（P0.05）.Conclusion Immune function of HHC patients can be improved by the treatment of RFA.