舌鳞状细胞癌的基因表达谱分析

Analysis of Gene Expression in Tongue Squamous Cell Carcinoma by cDNA Microarray

目的:探讨舌鳞状细胞癌的候选基因。方法:采用包含有4096个cDNA克隆的基因芯片技术,分别对3例舌鳞状细胞癌组织及其相应正常组织进行基因表达谱的比较。结果:3例标本共同表达的差异表达基因共126条,其中Ratio>2的明显上调基因11条,而Ratio<0.5的明显下调基因6条。结论:生物信息分析显示:显著差异表达基因与肿瘤的发病机理存在相关性。

Objective： To study the candidate genes of tongue squamous cell carcinoma by the method of microarray.Methods： We study novel gene expression by cDNA microarray method.The total RNA from both 4 cases of tongue squamous cell carcinoma tissues and normal tongue squamous cell tissue were compared.The PCR product of 4096 genes was spotted onto a kind of chemical-material-coated-glass slide in array.Then they were reversely transcribed to cDNA with the incorporation of fluorescent-labeled dCTP to prepare the hybridization probes.After hybridization,cDNA microarray was scanned for the fluorescent intensity.TSCC-related gene expression was screened through the analysis of difference in gene expression profiles.Results： Among all target genes,there were 126 genes whose expression level differed from TSCC and normal tissues.After we analyzed the genes,we found that 17 genes were obviously related to TSCC.Conclusion： Our results suggest that significantly differential expression of genes may be associated with the pathogenesis and progression of the TSCC.