XIAP、XAF1在口腔鳞癌癌周角质形成细胞中的表达与定位

Expression and Subcellular Localization of XIAP and XAF1 in Peri-tumoral Keratinocytes of Oral Squamous Cell Carcinoma

目的:明确人口腔鳞癌癌周角质形成细胞中XIAP与XAF1的表达与定位情况。方法:对人口腔鳞癌癌周角质形成细胞进行体外培养,间接免疫荧光染色结合激光共聚焦显微镜检测细胞中XIAP和XAF1蛋白的表达与亚细胞定位。结果:XIAP在口腔鳞癌癌周角质形成细胞中呈强阳性表达,胞浆和胞核均有分布,以胞核荧光染色更强。而XAF1主要分布于细胞核内,尤以核膜和核仁染色较强。结论:在口腔黏膜癌变过程中XIAP可能通过过度表达和亚细胞定位的变化而发挥重要的抗凋亡作用,而XAF1表达强度未发生明显变化,不能有效拮抗XIAP的作用。

Objective： To clarify expression and subcellular localization of XIAP and XAF1 protein in human oral keratinocytes from peri-tumoral dysplastic tissues of oral squamous cell carcinoma（OSCC）.Methods： The keratinocytes were harvested from peri-tumoral dysplastic tissues and cultured in vitro.Expression and subcellular localization of XIAP and XAF1 protein were analyzed by combination of indirect immunofluorescence and confocal laser scanning microscopy.Results： In the peri-tumoral keratinocytes,high level of XIAP protein could be detected in both the cytoplasm and the nucleus,with majority of XIAP packed in the nucleus.In the hNOKs,XAF1 distributed mostly in the nucleus,especially dense in the nuclear envelope and the nucleolus.Conclusion： In cancerization of oral mucosa,XIAP protein could play important antiapoptotic role by overexpression and changed subcellular localization,while level of XAF1 protein did not appear to be upregulated and XAF1 could not antagonize effectively the role of XIAP.