摘要
In order to better understand shrimp allergen,some basic characters of the major allergen of greasy-back shrimp(Metapenaeus ensis)were investigated.The major allergen was extracted and separated,and its peptide mass fingerprint(PMF)wasanalyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).After screening inthe NCBI database with Mascot searching engine,the results indicated that the major allergen of greasy-back shirmp was muscletropomyosin.Database matching search showed that the top protein matched,i.e.the tropomyosin from giant tiger prawn(Penaeusmonodon),had a Mowse value of 268.In addition,there were 27 queries matched with the allergen in greasy-back shirmp with anamino acid sequence coverage value of 65%.The matching scores and the sequence coverage values were also high with tropomy-osins of other invertebrates,including Tyrophagus putrescentiae and Lepisma saccharina.These results indicated that the allergen ofMetapenaeus ensis had high homology with other crustacean allergens,and provided molecular explanations for the highcross-reactivity of the major allergens between crustaceans and some other invertebrates.
In order to better understand shrimp allergen, some basic characters of the major allergen of greasy-back shrimp （Metapenaeus ensis） were investigated. The major allergen was extracted and separated, and its peptide mass fingerprint （PMF） was analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS）. After screening in the NCBI database with Mascot searching engine, the results indicated that the major allergen of greasy-back shirmp was muscle tropomyosin. Database matching search showed that the top protein matched, i.e. the tropomyosin from giant tiger prawn （Penaeus monodon）, had a Mowse value of 268. In addition, there were 27 queries matched with the allergen in greasy-back shirmp with an amino acid sequence coverage value of 65%. The matching scores and the sequence coverage values were also high with tropomy- osins of other invertebrates, including Tyrophagus putrescentiae and Lepisma saccharina. These results indicated that the allergen of Metapenaeus ensis had high homology with other crustacean allergens, and provided molecular explanations for the high cross-reactivity of the major allergens between crustaceans and some other invertebrates.
基金
supported by the Nature Science Foundation of China(No.30800859 and No.30871948)
the Key Projects in the National Science and Technology Pillar Program during the Eleventh Five-year Plan Period(2008BAD94B09)
