小型猪复合麻醉剂对大鼠不同脑区cAMP信号转导系统的动态影响

Dynamic effect of the compound anesthetic for miniature pigs on cAMP signal transduction system in different brain regions of SD rats

将252只SD大鼠随机分为对照组和低、高剂量组(分别腹腔注射生理盐水10 mL/kg及小型猪复合麻醉剂5 mg/kg、7.5 mg/kg),每个剂量组又分为麻醉组、恢复Ⅰ组和恢复Ⅱ组等3个亚组。用放射免疫法测定不同脑区的AC活性、cAMP含量和PDE活性。结果显示,低剂量小型猪复合麻醉剂基本上对cAMP信号转导系统无明显的作用,高剂量小型猪复合麻醉剂不仅使大鼠大脑、小脑、海马及丘脑等脑区AC活性、cAMP含量明显升高,而且还使PDE活性显著降低,并在此四部分脑区体现出系统性、高度相关性及剂量依赖性。表明,大鼠大脑皮层、小脑、海马、丘脑等脑区的cAMP信号转导系统可能参与了小型猪复合麻醉剂全麻机理的调控,该制剂的全麻作用与激活此四部分脑区的cAMP信号转导系统相关。

252 SD rats were divided randomly into control group（normal saline 10mL/kg ip）,high dose group（the compound anesthetic 5mg/kg ip） and low dose group（the compound anesthetic 7.5mg/kg ip）.Both the high dose group and the low dose group were further divided into anesthesia group,recoveryⅠgroup and recoveryⅡ group,respectively.Activities of AC and PDE and content of cAMP were measured by radioimmunoassay.In result,low dose compound anesthetic had not obvious effect on cAMP signal transduction system,but high dose compound anesthetic could enhance activity of AC and content of cAMP but also decrease activity of PDE in cerebral cortex,cerebellum,hippocampus and thalamus.And there was a systemic,dose-dependent and high correlation in these brain regions.In conclusion,cAMP signal transduction system in cerebral cortex,cerebellum,hippocampus and thalamus might participate in regulation molecular mechanisms of compound anesthetic general anesthesia,and general anesthetic effect of combined anesthetic might correlate with activation cAMP signal transduction system in these brain regions.