IFN-γ逆转免疫编辑后人肺癌A549细胞对CIK杀伤敏感性的研究

Studies on IFN-γ-mediated reversion of CIK killing sensitivity to edited human lung cancer A549 cells

目的:探讨IFN-γ对免疫编辑后人肺癌A549细胞对CIK杀伤敏感性的逆转作用。方法:应用RT-PCR和MTT方法分别检测IFN-γ对免疫编辑后A549细胞表面MICA表达的影响及A549细胞对CIK杀伤敏感性的变化。结果:免疫编辑后A549细胞表面低表达MICA,对CIK细胞杀伤敏感性低。IFN-γ可明显上调编辑后A549细胞MICA mRNA的表达率,提高CIK对编辑后A549细胞的细胞毒活性。结论:IFN-γ可以增强A549细胞表面MICA mRNA表达,逆转免疫编辑后A549细胞对CIK细胞杀伤敏感性。

AIM：To explore IFN-γ-mediated reversion of CIK killing sensitivity to immunoedited lung cancer A549 cells. METHODS：RT-PCR and MTT methods were used to detect the effect on MICA mRNA expression induced by IFN-γ in the edited A549 cells and the change of CIK killing sensitivity to A549 cells,respectively. RESULTS：Low expression of cell surface MICA and low killing sensitivity of CIKs were observed in edited A549 cells. IFN-γ could significantly increase MICA mRNA expression in the edited A549 cells and improve CIK cell cytotoxicity. CONCLUSION：IFN-γ could reverse CIK killing sensitivity to the edited A549 cells by enhancing the MICA mRNA expression.