潜在血源传播患者梅毒血清学TRUST/TPPA与IgM抗体联合检测

Serological Detection by Combination of TRUST with TPPA and IgM Antibody for Syphilis Patients with Latent Blood-transmitted Risk

目的为潜在血源传播患者筛选梅毒血清学传染病指标。方法采用回顾性研究,对2006年6月1日～2009年12月31日本院23720例潜在血源传播患者与门诊患者的TRUST/TPPA联合检测结果进行分析比较;分析不同年度潜在血源传播患者TRUST-/TPPA+的百分率变化;对其中118份TRUST-/TPPA+的潜在血源传播患者血清标本同时采用TPPA法和FTA-ABS法进行Tp-IgM抗体检测。结果潜在血源传播患者和门诊患者的梅毒抗体阳性率分别为8.62%和26.69%,二者差异有显著性;在梅毒抗体阳性标本中,TRUST-/TPPA+占有相当的比例,二者分别为3.12%和7.50%;潜在血源传播患者中TRUST-/TPPA+的百分率呈逐年上升趋势;118份TRUST-/TPPA+的潜在血源传播患者血清,经TPPA法和FTA-ABS法检出TP-IgM抗体阳性分别为13例和10例。结论潜在血源传播患者的梅毒传染性指标的监测应至少包含特异性抗体试验,如TPPA;TP-IgM检测有助于判断其传染性。

Objective To select serological screening indicators for syphilis patients with latent blood-transmitted risk. Methods A retrospective study on the TRUST and TPPA combination detection method was conducted between patients at latent blood-transmitted risk and outpatients in our hospital from, 1 June ,2006 to 31 December,2009. The percent of TRUST-/TPPA ＋ in patients with latent blood-transmitted risk were analyzed in different years. Tp-IgM antibodies were detected in 118 cases of TRUST-/TPPA ＋ patients at latent blood-transmitted risk. Results Syphilis antibody positive rate （26.69%） of outpatients was significantly higher than patients with latent blood-trans-mitted risk （8.62%）. The rate of TRUST-/TPPA ＋ in the syphilis-positive patients were 7.50% and 3.12%. The percent of TRUST-/TPPA ＋ in patients with latent blood-transmitted risk is growing steadily year after year. 13 and 10 IgM antibody positive specimens were seeked out of the 118 latent blood-transmitted risk TRUST-/TPPA ＋ sera by TPPA and FTA-ABS methods. Conclusions Serological screening for infectious syphilis should at least cover specific antibody tests, such as the TPPA; TP-IgM test would help determine contagiousness.