细胞因子对树突状细胞抗肝癌作用的影响

Effects of cytokines on dendritic cells against human hepatoma cell line *

目的研究人血树突状细胞（ＤＣ）和细胞因子ＴＮＦ，ＧＭＣＳＦ或ＩＦＮγ联合ＤＣ对淋巴因子和ＰＨＡ激活的杀伤细胞（ＬＰＡＫ细胞）体外杀伤人肝癌细胞株ＢＥＬ７４０２的影响．方法实验分为Ｌ组（ＬＰＡＫ），Ｄ组（ＬＰＡＫ＋ＤＣ），Ｔ１组（ＬＰＡＫ＋ＤＣ＋ＴＮＦ５０００ｋＵ／Ｌ），Ｔ２组（ＬＰＡＫ＋ＤＣ＋ＴＮＦ５００ｋＵ／Ｌ），Ｇ１组（ＬＰＡＫ＋ＤＣ＋ＧＭ－ＣＳＦ５００ｋＵ／Ｌ），Ｇ２组（ＬＰＡＫ＋ＤＣ＋ＧＭ－ＣＳＦ１００ｋＵ／Ｌ），Ｉ１组（ＬＰＡＫ＋ＤＣ＋ＩＦＮγ５００ｋＵ／Ｌ）和Ｉ２组（ＬＰＡＫ＋ＤＣ＋ＩＦＮγ１００ｋＵ／Ｌ）．每组效靶细胞比分别采用５∶１和１０∶１两种．培养４８ｈ后用中性红比色法检测细胞毒活性．结果Ｌ，Ｄ，Ｔ２和Ｔ１组的细胞毒活性依次增强，各组间差异有显著性（Ｐ＜００１）．Ｇ１和Ｇ２组均高于Ｄ组（Ｐ＜００１），但Ｇ１，Ｇ２组间差异无显著性．Ｉ１，Ｉ２组与Ｄ组相比，也无显著性差异．随效靶比增加，各组细胞毒活性均相应增强．结论ＤＣ能增强ＬＰＡＫ细胞对肝癌细胞ＢＥＬ７４０２的细胞毒活性；ＴＮＦ或ＧＭＣＳＦ与ＤＣ联用，两者有协同作用；但与ＩＦＮγ联用。

AIM To study the effects of human peripheral blood dendritic cells (DC) with or without TNF, GM CSF or IFN γ on lymphokine and PHA activated killer (LPAK) cells activity on human hepatoma cell line BEL 7402 in vitro . METHODS The experiments were divided into eight groups: L group (LPAK), D group (LPAK+DC), T1 group (LPAK+DC+TNF 5000kU/L ), T2 group (LPAK+DC+TNF 5000kU/L ), G1 group (LPAK+DC+GM-CSF 500kU/L ), G2 group (LPAK+DC+GM-CSF 100kU/L ), I1 group (LPAK+DC+IFN-γ 500kU/L ), I2 group (LPAK+DC+IFN-γ 100kU/L ), with two ratios of effect and target (5∶1 and 10∶1) cells in each group. After culture for 48 hours, the cytotoxicity was measured with neutral red assay. RESULTS The cytotoxic activity was sequentially enhanced from L, D, T2 to T1 group, the differences between groups were statistically significant ( P <0 01). The cytotoxic activity in both group G1 and G2 was significantly higher than that in group D ( P <0 01), but it had no difference between group G2 and G1 ( P >0 05). There was no significant difference in cytotoxic activity among group I1,I2 and D ( P >0 05). The cytotoxic activity was increased with the increase of the ratio of effect and target cells in all groups. CONCLUSION DC can enhance cytotoxic activity of LPAK cells on BEL 7402; TNF or GM CSF and DC synergistically enhance LPAK cell activity but no significant increase of LPAK cell activity with IFN γ and DC cooperation.