24种食蟹猴MHC B座位等位基因序列特征与进化分析

Identification of 24 MHC-B Allies in Local Cynomolgus Macaque and Ananlysis of Anagenesis

食蟹猴(Macaca fascicularis,Mafa)是重要的医学研究动物模型,被大量用于药物及移植医学试验。为探讨本地食蟹猴MHC I类基因B座位的多态性,本研究经大量PCR,克隆与测序,从10只食蟹猴中分离到24种Mafa-B等位基因的全长序列,其中4种为新序列,已递交NCBI数据库,并给予系统命名。在每个个体中均获得6至11条Mafa-B等位基因,显示食蟹猴的MHC-B座位出现扩增,食蟹猴MHC-B为重复基因座的特征与恒河猴类似。氨基酸序列比对分析得出,MHC-B基因在抗原肽结合区高度多态。

cynomolgus macaque is an important biomedical research animal model,and local cynomolgus macaque has been widely utilized in pharmaceutical and surgery studies. In order to explore the polymorpbism of MHC I gene B locus in local cynomolgus macaque,we obtained 24 M HC-B alleles in 10 cynomolgus macaque peripheral blood,moreover,4 M HC-B alleles were newly discovered in research,which have been send to NCBI wcbsite and been named by Immuno Polymorphism Database Non-Human Primate （NHP）Sequences, it was probable that Mafa-B gene was duplicated,because each sample cynomolgus contained average 6-11 alleles,which like the Mamu-B gene in macaca mamulatta.