腹腔高渗热化疗对荷瘤裸小鼠正常组织的影响

EFFECT OF PERITONEAL PERFUSION WITH HYPERTHERMIA, HYPER OSMOLAR SOLUTIONS AND ANTINEOPLASTIC DRUG ON NORMAL TISSUE IN NUDE MICE

为观察荷瘤裸小鼠在腹腔高渗热化疗灌注后正常组织的病理变化，选取５０只鼠龄７～１０周的ＢＡＬＢ／ｃｎｕｎｕ腹腔荷瘤裸小鼠，分组行７．５％ＮａＣｌ，５μｇ／ｍｌＭＭＣ，４２℃／３０ｍｉｎ持续腹腔灌注，取肝、肾、脾、回肠组织，常规ＨＥ染色后光镜观察形态学变化。结果：高渗及高渗化疗组肝、脾、肾无变化；高渗化疗组大肠浆膜下轻度充血水肿；温热及高渗热化疗组肝细胞轻度嗜酸性变性、脾充血、肾小管及集合管轻度水样变性；温热组小肠全层充血，绒毛轻度水肿；高渗热化疗组则可见部分绒毛顶部细胞脱落，陷凹细胞正常。

To observe the pathologic changes of normal tissue in nude mice after peritoneal perfusion with hyperthermia, hyper osmolar solution and mitomycin C (MMC). Fifty BALB/c nunu mices (7-10 weeks old) bearing HT 2 lines were chosen for the study, and were randomly divided into five groups: isotonic solution (control group), hyper osmolar solution (HOS group), HOS plus MMC group, hyperthermia (HT group) and HOS plus HT plus MMC group. After continuous hyperthermic peritoneal perfusion (42℃/30min) with 7.5% NaCl and 5μg/ml MMC, the liver, spleen, small intestine and kidney were examined by light microscopy. Results: ①In HOS and HOS plus MMC groups, no changes of liver, spleen and kidney were found. ②In HT and HOS plus HT plus MMC groups, slight degeneration of liver, hyperemia of spleen, swelling of kidney tubule cells and small intestine were found. ③In HOS plus HT plus MMC group, partial loss of small intestinal villi were also observed. Conclusioin: After continuous hyperthermic perfusion conbined with hyper osmolar solutions and mitomycin C, a slight injury was showed in normal tissue of nude mice.