p53和ras癌基因表达产物联合检测在胃癌早期诊断中意义

Combining detection expression of p53 and ras gene in gastric cancer and its clinical significance

目的：为了研究ｐ５３和ｒａｓ癌基因表达产物（ｐ５３蛋白和ｐ２１ｒａｓ、ｐ２１蛋白）在胃癌早期诊断中的价值。方法：对２２例浅表性胃炎，２５例重度不典型增生，３０例早期胃癌标本进行ｐ５３和ｐ２１蛋白免疫组化检测。结果：在浅表性胃炎中两种蛋白均为阴性：重度不典型增生和早期胃癌中两种蛋白阳性率非常接近，其差异性不显著（Ｐ＞００５），但均高于浅表性胃炎，差异性显著（Ｐ＜００５）。ｐ５３和ｐ２１蛋白联合检测阳性率，在重度不典型增生（７６０％）和早期胃癌（７００％）中，均高于两组中ｐ５３蛋白单项阳性率（４００％和３６７％）和两组中ｐ５３和ｐ２１蛋白同时阳性率（２４０％和２６７％）其差异性均显著（Ｐ＜００５）。结论：重度不典型增生在ｐ５３和ｒａｓ基因表达水平上接近早期胃癌，二种基因表达产物联合检测在胃癌早期诊断中有临床应用价值。

Objective: To investigate the relationship between the overexpression of p21 ras, p21 protein and p53 protein, and the value to the early diagnosis of the gastric cancer.Methods: 30 cases of early gastric cancer, and 25 cases of severe dysplasia were selected as the objects of study, and 22 cases of superficial gastritis were taken as control in this study. Results: There was significant differnce between gastritis and severe dysplasia and early gastric cancers( P <0 05), but there were insignificance difference between severe dysplasia and early gastric cancer( P >0 05). Combining-detection positiveness was 76.0% in the severe dysplasia, 70.0% in the early gastric cancer.all significantly higher than the positive rates: 40.0% and 36.7% of the p53 protein ,and 24.0% and 26.7% of the positiveness of both p53 and p21 protein in the severe dysplasia and early gastric cancer( P <0 05).Conclusion: The positive exprssions of p53 and ras gene were similar between those in the severe dysplasia and early gastric cancers. The clinical value of p53 and p21 protien combining detection was higher in the early diagnosis of gastric canaer.