HPLC法测定醋酸氯己定子宫灌注液的含量

Determination of Chlorhexidine Acetate in Uterus Perfusion Agent by HPLC

建立了测定醋酸氯己定含量的高效液相色谱法。以C18(4.6 mm×150 mm,5μm)为色谱柱,0.02 mol/L磷酸二氢钾溶液(pH2.6)-乙腈(70∶30)为流动相,流速1 mL/min,检测波长254 nm。结果显示,醋酸氯己定在0.5～198μg/mL浓度范围内,浓度对峰面积的线性关系良好(r=0.999 98),平均回收率为96.40%(n=9)。实验表明,该方法灵敏、准确,可以较好地控制醋酸氯己定子宫灌注剂的质量。

An HPLC method was established for the determination of chlorhexidine acetate in uterus perfusion agent.The analysis was carried on a column of C18（4.6 mm×150 mm,5 μm）,using 0.02 mol/L KH2PO4（pH2.6）-acetonitrile（70∶30）as the mobile phase with a flow rate of 1 mL/min,and UV detection at 254 nm.Results showed that a good linearity can be found in the range of 0.5～198 μg/mL（r=0.999 98）.The average recovery was 96.40%（n=9）.This method was more accurate and sensitive,and can be used to control the chlorhexidine acetate in uterus perfusion agent.