野马追对急性油酸性肺损伤大鼠肺血管通透性的影响

Influence of Eupatorium Lindleyanum on Pulmonary Vascular Permeability of Oleic-Induced Acute Lung Injury in Rats

目的探讨中药野马追对急性油酸性肺损伤大鼠肺血管通透性的影响。方法将72只Wistar大鼠随机均分为空白对照组,模型对照组,地塞米松组,野马追大剂量组、中剂量组和小剂量组,试验前2周各组大鼠灌服相应的药物,然后以油酸0.1mL/kg静脉注射复制急性肺损伤模型,光镜观察肺部组织形态学变化,称重法计算肺系数,紫外分光光度法检测肺组织微血管的渗透性和支气管-肺泡灌洗液(BALF)中蛋白含量并进行渗出细胞计数。结果野马追能明显降低BALF中渗出细胞数、蛋白含量、肺系数及肺含水量和肺血管通透性。结论中药野马追对急性肺损伤有防治作用,可能是通过降低急性肺损伤时肺血管通透性来实现的。

Objective To investigate the influence of Eupatorium lindleyanum on pulmonary vascular permeability of oleic-induced acute lung injury in rats.Methods 72 Wistar rats were randomly divided into six groups：high dose,medium dose and low dose of Eupatorium lindleyanum treatment groups,control group,model group and dexamethasone group.Two weeks before experiment,all groups were drenched with corresponding drugs.The acute lung injury model was established in the rats by intravenous injection of oleic acid（0.1 mL/kg）.Light microscope was used to examine the histological changes and lung index as well as wet to dry weight ratio was calculated by weighting method.Lung vascular permeability and protein level in BALF were detected by ultraviolet spectrophotometry.Results The oleic-induced elevation of lung index was restrained,as well as wet to dry weight ratio,lung vascular permeability and protein level in BALF in Eupatorium lindleyanum groups.Light microscope findings showed that the impairment in Eupatorium lindleyanum groups was far less severe than that in the model groups.Conclusion Pretreatment of Eupatorium lindleyanum shows the beneficial effect on acute lung injury induced by oleic acid in rats.The decrease in pulmonary vascular permeability may be involved in the mechanism of action of Eupatorium lindleyanum effects.