浙江省近年H_3N_2亚型流行性感冒病毒流行株的血凝素与神经氨酸酶基因与猪型流行性感冒病毒的亲缘关系分析

Phylogenetic Analysis Based on Hemagglutinin and Neuraminidase Genes between Swine Influenza Virus and Human Influenza A/H_3N_2 Virus Strains Isolated in Zhejiang Province

目的对浙江省近年两次甲3亚型(H3N2)流行性感冒(流感)病毒流行的代表株,与猪型H3N2株流感病毒在主要抗原基因间进行比较分析,探讨二者间的亲缘关系。方法对这两次流行流感病毒代表株的血凝素(Hemagglutinin,HA)与神经氨酸酶(Neuraminidase,NA)基因作序列测定,并与同期的人、猪流感毒株的相应基因作同源性与进化树分析。结果浙江省这两次H3N2流感流行中的代表株甲(3A3)/浙江(Zhejiang,ZJ)/10/98、A3/ZJ/6/99、A3/ZJ/8/02与猪流感病毒A3/猪型(SW)/安大略(Ontario,ON)/130/97、A3/SW/香港(Hongkong,HK)/4361/99、A3/SW/HK/74/02在HA1区的同源性,分别为99.1%、99.4%、99.4%;在NA区,A3/ZJ/10/98、A3/ZJ/6/99、A3/ZJ/8/02与猪流感病毒A3/SW/ON/130/97、A3/SW/HK/4361/99、A3/SW/HK/74/02的同源性,分别达到98.2%、99.3%、99.3%。二者之间均呈现出很高的同源性,有的远高于它与同期人流感毒株的同源性,在基因系统进化树上也呈现出这一状况。结论浙江省这两次H3N2流感流行的毒株与猪流感的某些毒株之间存在着密切的联系,猪流感与人流感之间的关系值得深入研究。

Objectives To investigate the phylogenetic relationship between swine influenza A/H3N2 virus and the representative strains of human influenza A/H3N2 virus isolated in two epidemics in recent years through comparing the sequences within HA and NA genes.Methods HA and NA gene of the human representative strains were sequenced,and then phylogenetic tree with the swine and human strains isolated in the corresponding period of time were constructed.Results The homologies on the HA1 domain between human representative strain（sA/Zhejiang/10/98,A/Zhejiang/6/99 and A/Zhejiang/8/02）and the swine strains （A/SW/Ontario/130/97、A/SW/Hongkong/4361/99 and A/SW/Hongkong/74/02）were 99.1%,99.4%and 99.4%respectively.Based on the NA gene,the homologies between human strain（sA/Zhejiang/10/98,_A/ Zhejiang/6/99 and A/Zhejiang/8/02）and the swine strains（A/SW/Ontario/130/97,A/SW/Hongkong/4361/99 and A/SW/Hongkong/74/02）were 98.2%,99.3%and 99.3%respectively.The results showed that the two types of influenza viruses were highly homologue,and even some of their homologies were higher than that amongst the contemporary human influenza A/H3N2 strains.The same results shown in the phylogenetic tree. Conclusions The human influenza A/H3N2 virus isolates in the two epidemic closely associated with some of the swine influenza virus strains,and their relationship should be further studied.