山地乌骨鸡不同组织中CAPN 1基因表达的发育性变化

Developmental changes of CAPN 1 gene expression in tissues of mountain black-bone chicken

本试验采用SYBR GreenⅠ荧光定量法,首次测定了四川山地乌骨鸡1d、14d、28d、42d、56d、70d、84d的胸肌、腿肌、肝脏、心、脑组织中钙蛋白酶1CAPN1基因的相对表达量。结果表明:CAPN1基因在鸡体内普遍存在,CAPN1基因在肌肉组织中的表达量与鸡肌肉组织的生长发育呈相似的变化规律。

We adopted the real-time PCR method to test the relative quantity of CAPN 1 gene in breast muscle,leg muscle,liver ,heart and brain of Sichuan Mountainous Black-bone chicken at 7 growth points,including 1 day,14 days,28 days,42 days,56 days,70 days and 84 days. The results indicated that CAPN 1 was a ubiquitous protein in chicken,and the mRNA expression regulation of CAPN 1 gene in muscle was similar to the growth regulation of chicken muscle.