麻醉剂MS-222对金鱼体内AKP、CAT和ACP活性的影响

Effects of the anesthetic MS-222 on the AKP,CAT and ACP activities in goldfish

在实验室条件下,采用4个浓度0mg/L、30mg/L、60mg/L和90mg/L,分析了麻醉剂MS-222对金鱼鳃、肝脏和肌肉组织中碱性磷酸酶(AKP)、酸性磷酸酶(ACP)和过氧化氢酶(CAT)活性的影响。试验结果表明:ACP明显出现一个先增后降的趋势,呈抛物线状;在低浓度(≤60mg/L)下,酶活性在各组织中呈现一定的稳定状态,而当麻醉剂浓度升高和麻醉时间增长,酶活性逐渐下降。AKP在低浓度麻醉剂溶液中,酶活性有明显的增加,随着浓度的升高(≥60mg/L)和时间的增长,酶活性呈抑制状态。而在肌肉中这种情况并不明显。CAT在一定阶段(30mg/L～60mg/L)呈现稳定的状态、而后抑制逐渐明显,当麻醉剂MS-222浓度达到一定量(≥60mg/L)以后,对鱼体的刺激就不再增加了,酶活性也相对处于较稳定的状态;当麻醉剂随着时间的增长和浓度的增大时,在鱼体内富集的麻醉剂破坏鱼体内的过氧化氢酶的分子构象,使酶体失去活性。

The effect of the anesthetic MS-222 on the activities of alkaline phosphatase（AKP）,catalase（CAT）and acid phosphatase（ACP）in goldfish was analyzed under laboratory conditions.Four concentrations 0 mg/L,30 mg/L,60 mg/L and 90 mg/L of MS-222 were tested.The results showed that the concentration of MS-222 affected the activities of AKP,CAT and ACP in the gill,liver and muscle of goldfish.The results show that the activity of alkaline phosphatase is first increased and then decreased,shows a parabola curve which activity change;the activity is stable under the lower anesthetic concentration（≤60 mg/L）,decreased with increasing of concentration and longer induction time in tissue.The activity of AKP significantly increased under the low anesthetic concentration.Decreased with increasing of concentration（（≥60 mg/L）and longer induction time in tissue,but in muscle is not as in tissue.In the concentration（30 mg/L^60 mg/L）,the activity of CAT is stable at first;when the anesthetic concentration higher than 60 mg/L,no further irritation happen.With the increasing concentration and time,fish accumulate the anesthetic to lead to the conformation damage and then deactivation.