非共轴激光共焦显微技术的研究现状与展望

Review and Expectation of Non-Coaxial Laser Confocal Microscopy

非共轴激光共焦显微技术是近年来激光共焦显微成像领域发展起来的一种新型成像方法。该成像方法将被测样品与照明光轴成一定角度放置,使得照明光轴与采集光轴形成一定的夹角,减小系统合成焦体体积、提高轴向分辨力。与传统的共焦显微技术相比,该方法可以有效兼顾激光共焦显微系统的轴向分辨力、工作距和视场大小。介绍了非共轴激光共焦显微技术的成像原理,详述了共焦theta显微技术和双轴共焦显微技术这两种典型的非共轴激光共焦显微技术的研究现状和发展趋势,简述了在双轴共焦显微技术领域的研究设想。

Non-coaxial laser confocal microscopy is a novel imaging method of laser confocal microscopy.By observing the sample at an angle to the illumination axis,this method decreases the observation volume of overlap and then improves the axial resolution.Compared with the conventional confocal microscopy,this method has gained wide attention for its advantage that it can improve the resolution,working distance and field of view simultaneously.We summarize the key principle of the non-coaxial laser confocal microscopy,and expand the recent progress and development trend of two typical non-coaxial laser confocal microscopies,confocal theta microscopy and dual-axes confocal microscopy.Finally we briefly introduce our researching plan.