马齿苋多糖对雏鸡免疫器官内细胞增殖的影响

The effects of POP on cell proliferation in immune organ of chicken

本研究在不同剂量(0、25、50mg/kg和100mg/kg)马齿苋多糖(POP)作用雏鸡后,通过流式细胞仪检测其免疫器官细胞增殖情况,并称免疫器官重,计算免疫器官指数。结果表明,随着POP剂量的增加,除50mg/kg与100mg/kgPOP剂量组法氏囊指数升高,差异不显著(P>0.05)外,其他雏鸡免疫器官指数和细胞增殖指数显著升高(P<0.05)。细胞增殖指数在雏鸡免疫器官内均以G1/G0期细胞为主,少量细胞为S期和G2/M期,且不同免疫器官细胞周期变化相似。这说明,POP能促进雏鸡免疫器官的生长发育和雏鸡免疫器官内细胞增殖,增强免疫功能,提高机体的免疫力。

The objective of this research was to study the change of immune organs indexes and cell proliferation in immune organs of chicken by polysaccharide from portulaca oleracexa（POP）（0,25,50 mg/kg and 100 mg/kg）with flow ey- tometry.The result showed that：with the increase of POP,immune organs indexes and the proliferous indexes （P I） in im- mune organs of chicken were increased（P 〈 0.05） ,except the bursa indexes between 50 mg/kg and 100 mg/kg（P 〉 0.05）. The majority of cells,in different immune organs of chickens were in Gl/G0 phase,and a few in S phase and G2/M phase. The changes of cell cycles in different immune organs were similar to each other.The conclusion was that：POP could promote the growth and cell proliferation in immune organs of chicken, enhanced the body immune function and immunity.