真菌激发子诱导虫草菌素高产的条件

Inducing Conditions of Fungal Elicitor on Cordycepin Acceleration

借鉴真菌激发子诱导植物细胞悬浮培养中次生代谢产物富积的理论、方法，筛选出对蛹虫草（Ｃｏｒｄｙｃｅｐｓｍｉｌｉｔａｒｉｓ）有拮抗或互生关系的真菌菌株并制备激发子粗提物。经平板纸片法和液体培养法测试表明，多数菌株对蛹虫草菌丝生长有较好的影响。建立和测定了蛹虫草生长曲线及真菌激发子处理的有效时间。测定和制作了检测真菌激发子诱导效果的虫草菌素标准曲线，其线性范围为０～４０μｇ／ｍｌ。

Based on the theory and methods used fungal elicitors to induce accumulation of some secondary metabolic products accumulation in plant suspended cell cultivation, we have selected several kinds of fungal strains which are antagonistic or syntrophic to Cordyceps militaris and prepared the crude elicitors. It was showed that a few of fungi had better affect to the C.militaris growth using plate paper and liquid cultivation. A growth curve of C.militaris and the effective treating time of some fungal elicitors have been determined and set up. The standard curve of cordycepin have been determined and drawn under the range of 0～40μg/ml. It can be used as analyzing criterion for checking the affection of fungal elicitors.