胆固醇结石患者肝脏核受体基因蛋白的表达

Expressions of liver nuclear receptor genes in patients with cholesterol gallstone disease

目的探讨胆囊胆固醇结石(CGS)患者肝脏的核受体基因的蛋白表达。方法对20例CGS(胆石组)和10例无胆石症的胆囊息肉患者(对照组)测定了胆石胆固醇成分及血清脂类成分:总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和胆汁脂类成分(胆固醇、磷脂和胆汁酸),并计算胆汁总脂和胆汁胆固醇饱和指数。Westernblot法测定肝脏肝受体同类物1(LRH-1)、法尼醇受体(FXR)、人类固醇异生物受体(SXR)及肝脏X受体a(LXRa)基因的蛋白表达量。结果胆石组血清HDL-C浓度明显低于对照组[(0.91±0.05)mmol/Lvs.(1.31±0.09)mmol/L](P<0.01);胆汁胆固醇摩尔百分比浓度较对照组高[(7.89±0.39)mol%vs.(5.15±0.89)mol%](P<0.01);胆汁总脂较对照组明显下降[(105.62±10.51)g/Lvs.(153.50±13.20)g/L](P<0.05);胆石组LRH-1蛋白表达高于对照组[(0.88±0.05)vs.(0.69±0.03)](P<0.05),LXRa、FXR和SXR表达两组差异无统计学意义(P>0.05)。结论人肝脏LRH-1的蛋白表达增高与胆囊胆固醇结石形成有关。

Objective To investigate the expressions of liver nuclear receptor genes in patients with cholesterol gallstone（CGS）disease.Methods The expressions of liver X receptor a（LXRa）,farnesoid X receptor（FXR）,steroid xenobiotic receptor（SXR）and liver receptor homolog-1（LRH-1）gene were detected by Western blot in 20 patients with CGS（group CGS）and 10 cases with cholesystis polypus without gallstones（group C）.Serum lipids,gallstone cholesterol and biliary composition were assayed as well.Cholesterol saturation index（CSI）in bile was calculated.ResultsCompared to group C,serum high density lipoprotein cholesterol was lower in group CGS than that in group C [（0.91±0.05）mmol/L vs.（1.31±0.09）mmol/L]（P0.01）.CSI was higher in group CGS than that in group C[（1.06± 0.03）vs（0.68±0.10）]（P0.01）.Biliary cholesterol was also higher in group CGS than that in group C [（7.89±0.39）mol% vs.（5.15±0.89）mol%]（P0.01）,while biliary total lipid was lower in group CGS than that in group C [（105.62±10.51）g/L vs.（153.50±13.20）g/L]（P0.05）.There were no significant differences in bile salts and lecithin between two groups.Expression of LRH-1 gene was higher in group CGS than that in group C [（0.88±0.05）vs.（0.69±0.03）]（P0.05）.There were no significant differences in protein expressions of LXRa,FXR and SXR gene between two groups.Conclusion CGS disease may be related to increased protein expression of LRH-1 gene.