动脉粥样硬化大鼠动脉组织中SSeCKS的表达

Expression of SSeCKS in aorta tissue in rats with atherosclerosis

目的研究动脉粥样硬化大鼠动脉组织中丝氨酸(Src)抑制的C激酶底物(SSeCKS)的表达。方法以高脂饲料喂养加维生素D3腹腔注射建立SD大鼠动脉粥样硬化模型(A),对照(B)组,每组8只给予基础饲料喂养。测定血清总胆固醇(TC)及甘油三酯(TG);病理学观察HE染色的主动脉;用免疫组织化学法及免疫印迹法检测SSeCKS在动脉中的表达,免疫荧光双标法观察SSeCKS的细胞定位。结果12周时A组TC和TG较B组明显升高(P<0.05);A组形成纤维增生性动脉粥样硬化病变,SSeCKS表达显著增强,SSeCKS表达主要分布在动脉内膜、中膜,部分定位于内皮细胞。结论高脂饮食所致动脉粥样硬化大鼠主动脉SSeCKS表达增加,SSeCKS可能参与了血管重塑及细胞凋亡从而促进动脉粥样硬化的发展。

Objective To investigate the expression of Src suppressed C kinase substrates（SSeCKS）in the atherosclerosis（AS）aorta of rats.Methods Sixteen male SD rats were randomly divided into two groups with 8 rats each.AS model was established by fat diet with vitamin D3 injection ip.in group A.The rats in group B were given standard diet as the controls.After 12 week,serum total cholesterol（TC）and triglyeride（TG）were detected.Thoracic aorta was stained with HE.SSeCKS expression was measured by immunohistochemistry,immunofluorescence and Western blot.Results Compared with group B,group A showed elevated TC and TG（P0.05）and aortic fibrous plaque formation.In group A,SSeCKS expression was significantly increased in the aorta and colabeled with endothelial cell marker CD31.Conclusion SSeCKS might influence vascular remodeling,which plays an important role in AS.