抑郁症患者外周血白细胞中CREB1基因表达研究

The study on the gene expression of cyclic AMP response element-binding protein in peripheral leucocyte from patients with major depression

目的探讨抑郁症患者外周血白细胞中cAMP反应元件结合蛋白(cyclic AMP response element-bind-ing protein,CREB)基因CREB1的表达情况。方法采用TaqMan实时定量反转录-聚合酶链反应(reverse tran-scription-polymerase chain reaction,RT-PCR)法,对36例首发未用药抑郁症患者及44名正常对照外周血白细胞中的CREB1基因表达水平进行定量分析。结果患者组和对照组的CREB1基因平均表达量(amole/0.3μLcDNA)分别为(0.20±0.10)和(0.17±0.06),差异无统计学意义(t=1.50,P>0.05)。根据性别、发病年龄、汉密尔顿抑郁量表-24项(HAMD-24)评分对患者进行分组,仍未发现患者组的基因表达量与对照组的差异有统计学意义(P>0.05),且与HAMD-24量表各因子分无相关(P>0.05)。结论抑郁症患者外周血白细胞中CREB1基因的表达水平未见异常,与性别、发病年龄和疾病严重程度无关。

Objective To investigate expression level of cyclic AMP response element-binding protein （CREB） gene,CREB1,in peripheral leucocyte from patients with major depression.Methods Thirty-six first-episode drug-naive depressive patients and 44 healthy controls were recruited.Relative TaqMan real-time quantitative reverse transcription-polymerase chain reaction （RT-PCR） of CREB1 gene in peripheral leukocyte was performed using the standard curve method on ABI PRISM 7900 sequence detection system.Amplification of an endogenous control,house-keeping glyceraldehyde 3-phosphate dehydrogenase（GAPDH） gene,was performed to standardize the amount of unknown samples.Furthermore,the relationship between CREB1 gene expression levels and gender,onset age,severity of depressive symptoms,as well as scores of HAMD-24 rating scale were analyzed.Results CREB1 gene expression level （amole/0.3 μL cDNA） in peripheral leucocyte from patients and controls was 0.20±0.10 and 0.17±0.06,respectively.There was no significant difference between two groups （t=1.50,P0.05）.Moreover,gene expression levels were not correlated with total scores and factor scores of HAMD-24 rating scales （P0.05）.Conclusions CREB1 gene expression level in peripheral leucocyte not different nor related to gender,onset age and symptom severity.