期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

脑膜炎奈瑟氏菌外膜蛋白NhhA的克隆表达及免疫学分析 被引量：4

Clone,Expression and Immunological Analysis of Neisseria meningitidis Outer Membrane Protein NhhA
原文传递
导出
摘要 通过克隆脑膜炎奈瑟氏菌NhhA基因GNA0992于原核表达载体pET-20b,在大肠杆菌BL21(DE3)中实现了可溶性表达,表达量约占菌体蛋白总量的20%。经初步纯化后免疫小鼠,对其免疫原性进行了初步分析。结果显示,经3次腹腔免疫,血清IgG滴度达到23186,同时杀菌力实验显示NhhA能诱导针对B群脑膜炎奈瑟氏菌的补体依赖的杀菌反应。证明了NhhA是一种良好的抗原,为疫苗开发的蛋白靶标筛选工作奠定了基础。 Neisseria meningitidis gene GNA0992 was cloned into the prokaryotic expression vector pET-20b,and soluble expressed in E.coli BL21 （DE3）.Purified NhhA stimulated a significant antigen-specific humoral response following three intraperitoneal immunization,while bactericidal assay showed that NhhA can elicit antibodies capable of inducing bactericidal activity against group B N.meningitidis strains.The results indicate that NhhA is a good candidate for vaccine development.
作者 陈柳 李健峰 徐维明 戴宗祥 李智华 孙强明
机构地区 北京协和医学院 中国医学科学院医学生物学研究所
出处 《中国生物工程杂志》 CAS CSCD 北大核心 2010年第4期8-12,共5页 China Biotechnology
关键词 脑膜炎奈瑟氏菌 NhhA 原核表达 免疫原性 Neisseria meningitides NhhA Prokaryotic expression Immunological property
分类号 Q786 [生物学—分子生物学]
  • 相关文献

参考文献10

  • 1Ashton F E, Ryan J A, Jones C, et al. Serotype of Neisseria meningitidis associated with an increased incidence of meningitis cases in the Hamilton area. Can J Microbiol, 1983, 29: 129- 130.
  • 2Henderson I R, Nataro J P. Virulence functions of autotransporter proteins. Infect Immun, 2001,69:1231-1243.
  • 3Scarselli M, Rappuoli R, Scarlato V. A common conserved amino acid motif module shared by bacterial and intercellular adhesins : bacterial adherence mimicking cell-cell recognition? Microbiology, 2001,147:250-252.
  • 4Peak I R A, Srikhanta Y, Dieckelmann M, et al. Identification and characterisation of a novel conserved outer membrane protein from Neisseria meningitidis. FEMS Immunol Med Microbiol, 2000, 28:329-334.
  • 5Pizza M, Scarlato V, Masignani V, et al. Identification of vaccine candidates against serogrou PB meningococcus by whole-genome sequencing. Science, 2000,287 : 1816-1820.
  • 6Scarselli M, Serruto D, Montanari P,et al. Neisseria meningitidis NhhA is a multifunctional trimeric autotransporter adhesin. Mol Microbiol, 2006, 61:631-644.
  • 7Sjolinder H, Eriksson J, Maudsdotter L. Meningococcal outer membrane protein NhhA is essential for colonization and disease by preventing phagocytosis and complement attack. Infection and Immunity. 2008, 76( 11 ): 5412-5420.
  • 8Frasch C E, Chapman S. Classification of Neisseria meningitides group B into distinct serotypes I. serologica typing by a microbactericidal method. Infect Immun, 1972, 5 ( 1 ) : 98-102.
  • 9Gold R, Lepow M L, Goldschneider I, et al. Immune response of human infants of polysaccharide vaccines of grou PA and C Neisseria meningitides. J Infect Dis,1977, 136 ( su PPI) : S31- 35.
  • 10Rouelle J L. Basb 029 polynucleotide(S) and polypeptides from Neisseria meningitidis. U.S. WO99/58683. 1999.

同被引文献30

  • 1Perrett K P, Pollard A J. Towards an improved serogroup B Neisseria meningitides. Vaccine ,2005,5 ( 12 ) : 1611-1625.
  • 2Murphy E, Andrew L, Lee K L. Sequence Diversity of the Factor H Binding Protein Vaccine Candidate in Epidemiologically Relevant Strains of Serogroup B Neisseria meningitides. J Infect Dis,2009, 200(3) :379-389.
  • 3Masignani V, Comanducci M, Giuliani M M. Vaccination against Neisseria meningitides Using Three Variants of the Lipoprotein GNA1870. J Exp Med,2003,197(6) :789-799.
  • 4Brunelli B, Del Tordello E, Palumbo E. Influence of sequence variability on bactericidal activity sera induced by Factor H binding protein variant 1.1. Vaccine,2011,29(5) : 1072-1081.
  • 5Pizza M, Donnelly J, Rappuoli R. Factor H-binding protein, a unique meningococcal vaccine antigen. Vaccine,2008,26 ( 8 ) : I46-I48.
  • 6Beemink P T, Granoff D M. Bactericidal Antibody Responses Induced by Meningococcal Recombinant Chimeric Factor H- Binding Protein . Vaccines,2008,76 (6) :2568-2575.
  • 7Beernink P T,Welsch J A,Bar-Lev M. Fine Antigenic Specificity and Cooperative Bactericidal Activity of Monoclonal Antibodies Directed at the Meningococcal Vaccine Candidate Factor H- Binding Protein. Infect Immun, 2008,76(9) :4232-4240.
  • 8Frasch C E, Chapman S S. Classification of Neisseria menitrgitidis group B into distinct semtypes. I. Serological typing by a microbactericidal method. Infect Immun,1972,5(1) :98-102.
  • 9Giuliani M M,Santini L, Brunelli B, et al. The region comprising amino acids 100 to 255 of Neisseria meningitides lipoprotein GNA 1870 elicits baeteficidal antibodies. Infect Immun ,2005,73 ( 2 ) : 3762-3771.
  • 10Schneider M C, Prosser B E. Neisseria meningitidis recruits factor H using protein mimicry of host carbohydrates. Nature ,2009,458 (7240) : 890-893.

引证文献4

二级引证文献5

中国生物工程杂志
2010年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部