摘要
以近亲个体‘顺10-16’、‘青北10-7’和‘贺春’为材料建立了两用桃AFLP研究体系,从64对引物组合中筛选出了E-AAT/M-ACT、E-AAT/M-CTG、E-ACA/M-CTG和E-ACA/M-CTT等4个多态性好、分辨率高的引物组合;应用该体系对‘锦春’等23个两用桃品种(系)进行AFLP分析,结果共扩增出127条带,其中多态性带62条,多态性百分率48.8%,以其中特异性较高的23个多态性条带构建了这23个两用桃品系的指纹图谱,为两用桃品种鉴定及保护奠定了基础。
To quickly identify new dual-purpose peach varieties and protect intellectual property rights,we constructed AFLP study systems of dual-purpose peach varieties with close relative individuals ＇Shun 10-16＇,＇Qingbei10-7＇ and ＇Hechun＇.Following an initial screening with 64 primer combinations,four primer pairs,E-AAT/M-ACT,E-AAT/M-CTG,E-ACA/M-CTG and E-ACA/M-CTT,which can produced high polymorphism and quality markers were selected.A total of 127 DNA fragment bands were amplified by using the 4 primer pairs,but only 62 bands were polymorphism among the 23 peach varieties.A fingerprinting map was constructed by using the 4 selected primer pairs of AFLP,which provide a platform for identification and protection of varieties in dual-purpose peaches.
出处
《西北植物学报》
CAS
CSCD
北大核心
2010年第3期505-511,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
北京市农业科技项目(20070613)
关键词
两用桃
AFLP
指纹图谱
品种鉴定
dual-purpose peach
AFLP
DNA fingerprinting
variety identification
