子痫前期患者胎盘骨桥蛋白及整合素α_νβ_3的表达及意义

Decreased Expression of Osteopontin and Integrin α_νβ_3 in the Placenta of the Patients with Preeclampsia

目的探讨骨桥蛋白(OPN)及其受体整合素ανβ3在子痫前期(preeclampsia,PE)患者胎盘组织中的表达及其意义。方法2008年11月-2009年9月,采用免疫组织化学方法检测20例PE患者(轻度及重度PE各10例)和14例正常足月孕妇(对照组)胎盘组织中OPN及ανβ3蛋白表达水平。采用RT-PCR检测各组孕妇胎盘组织中的OPN、αν和β3的mRNA的表达水平。结果PE组孕妇胎盘组织中OPN及ανβ3蛋白表达低下,与对照组相比,差异有统计学意义(P<0.05);重度PE组OPN及ανβ3蛋白表达水平更低,与轻度PE组比较,差异有统计学意义(P<0.05)。PE组孕妇胎盘组织中OPNmRNA水平明显低于对照组,两组差异有统计学意义(P<0.05);重度PE组OPNmRNA水平显著降低,与轻度PE组比较,两组差异有统计学意义(P<0.05);但αν和β3mRNA的表达水平三组间比较差异无统计学意义。结论OPN及其受体整合素ανβ3在PE胎盘组织中的低表达可能在子痫前期的发病过程中起重要作用。

Objective To study the mRNA and protein expression of osteopontin（OPN）and its receptor integrin ανβ3 in the placenta of preeclampsia patients and their roles on pathogenesis of preeclampsia.Methods Immunohistochemistry and RT-PCR were used to investigate the expression of OPN and ανβ3 protein and their mRNA in placenta of 20 women with preeclampsia（including 10 cases of mild preeclampsia,10 cases of severe preeclampsia）and 14 normal full term pregnant women respectively.Results The protein expressions of OPN and integrin ανβ3 were significantly decreased in the placenta from preeclampsia groups（P〈0.05）,and there was more significant difference between the severe and mild preeclampsia groups（P〈0.05）.Compared with the control group,the expression of OPN mRNA in preeclampsia groups was significantly lower（P〈0.05）,but there was no significant difference in the expression of αν and β3 mRNA among three groups（P〉0.05）.Conclusion The decreased expression of OPN and its receptor integrin ανβ3 may be a crucial role in the pathogenesis of preeclampsia,and they directly correlate with the level of preeclampsia.