干燥综合征抗原B基因在大鼠早期心肌缺血组织中的表达及其意义

Expression of SSB Gene in Rats with Early Ischemic Myocardium and Its Implications

目的探讨干燥综合征抗原B基因(SSB)在早期心肌缺血组织中的表达及其意义。方法健康SD大鼠随机分为手术组、假手术组和正常对照组,并将手术组心肌标本按缺血区和非缺血区又分为手术缺血组和手术非缺血组,手术组和假手术组老鼠根据设定的时间段又分为0 min、15 min、30 min、60 min和120 min 5个亚组,通过实时荧光定量PCR测定每个样本的目的基因的表达水平(mRNA),同时以大鼠β-actin基因片段作为内参对照以消除样本误差。结果 SSB基因表达在急性心肌缺血早期呈下调趋势,缺血120 min组SSBmRNA表达降低,与0 min组及正常对照组相比,差异具有统计学意义(P<0.05),其它组间SSBmRNA表达差异无统计学意义(P>0.05)。结论 SSB基因可能参与早期缺血心肌的病理生理的调节过程。

Objective To examine the expression of Sjren＇s syndrome antigen B（SSB） gene in rats with early ischemic myocardium.Methods Adult healthy Sprague-Dawley rats were randomly divided into four groups（ischemia（MI）,non-ischemia（NI）,sham-operated（SO） and normal control（NO） groups）.The expression of SSB and β-actin gene in the myocardium was examined by real time PCR at 0 min,5 min,15 min,30 min,60 min,120 min after ligation of the left anterior descending coronary artery（LAD）.Results The expression of SSB gene was down-regulated after ischemia.Significantly lower expression of the gene in the rats with myocardial ischemia was found at 120 min after ligation compared with those before ligation and the control group at 120 min after ligation（P〈0.05）.However,no significant differences were found between other groups（P〉0.05）.Conclusion SSB gene may be involved in the pathophysiologic regulating process in myocardial cells at the early stage of myocardial ischemia in rats.