全反式维甲酸对恶性黑素瘤A375细胞增殖及Fas蛋白表达的影响

Effect of All-trans Retinoic Acid on the Proliferation of and Fas Protein Expression in Human Malignant Melanoma A375 Cells

目的观察全反式维甲酸(ATRA)对体外培养恶性黑素瘤A375细胞增殖的抑制作用并探讨其作用机制。方法设置溶剂对照及ATRA不同浓度组,采用四甲基偶氮唑蓝(MTT)法检测ATRA不同浓度、作用不同时间各组细胞存活和生长情况,分析量效、时效关系;Western blot检测10μmol/L ATRA作用不同时间Fas蛋白的表达。结果 ATRA在0.01～0.1μmol/L浓度对A375细胞增殖抑制作用不明显;在1～100μmol/L浓度有抑制作用;药物作用72 h时各浓度组的细胞抑制率均达到高峰,以100μmol/L浓度组抑制率最高;10μmol/LATRA可上调A375细胞Fas蛋白的表达。结论 ATRA对A375细胞增殖的抑制作用呈时间和剂量依赖关系。ATRA可能通过Fas信号转导途径诱导A375细胞凋亡。

Objective To investigate the effect of all-trans retinoic acid on the proliferation of and Fas protein expression in malignant melanoma A375 cells in vitro.Methods Malignant melanoma A375 cells cultured under different doses of all-trans retinoic acid（ATRA） and solvent controls.The effect of ATRA on cell proliferation was observed.The growth rate of the cells was detected by MTT assay.The Fas protein expression was detected by Western blotting.Results ATRA inhibited the proliferation of A375 cells under 1-100 μmol/L of concentrations.The peak effect occurred after 72 hours of ATRA treatment,ATRA inhibited the growth of A375 cells in a dose and time-dependent manner.The level of Fas protein was up-regulated after exposure to 10 μmol/L of ATRA.Conclusion ATRA inhibits A375 cell proliferation perhaps through Fas death receptor pathway which induces A375 cell apoptosis.