以色列二穗短柄草遗传多样性分析

Genetic Diversity Analysis of Brachypodium distachyon from Israel

采用ISJ(Intron-Splice Junction,内含子切接点引物)分子标记方法对29份源自以色列的二穗短柄草(Brachypodium distachyon)及7份其他地区的二穗短柄草材料进行遗传多样性分析.结果表明:8个ISJ引物和10个引物组合共扩增出稳定条带145条,其中93条为多态性条带,多态性比率为64.58%.聚类分析将36份供试材料分成3大类,二倍体类型的二穗短柄草,六倍体类型源自以色列以外地区的二穗短柄草及来自以色列的六倍体二穗短柄草且以色列二穗短柄草材料进一步聚成不同生态环境下的两个亚类.主坐标分析结果与聚类分析结果基本吻合.研究结果为二穗短柄草的起源与分布提供了一定的理论依据,并为分子标记技术在二穗短柄草种质资源收集、评价和鉴定等方面的利用奠定了基础.

To investigate the genetic diversity among Brachypodium distachyon 29 accessions from Israel and 7 accessions from other areas,PCR with intron-splice junction primer was employed for this study.The results indicated that 145 distinct PCR bands in total were amplified using 8 primers and 10 primer combinations,and 93（64.58%） of them were polymorphic.A systematic cluster analysis conducted with UPGMA shows that 36 B.distachyon accessions can be classified into 3 groups：the first group being diploid accession,the second group being hexaploid not from Israel,the third group being a natural population from Israel,and this population was clustered into two different ecotype groups.Results obtained from the principal component analysis（PCA） were consistent with those from the cluster analysis.This study provided a theoretical foundation for origin and evolution of Israeli B.distachyon,collection and identification of genetic resource of this plant.