外源葡萄糖对莱茵衣藻生长和产氢的影响

Effects of Exogenous Glucose on Growth and Hydrogen Production of Chlamydomonas reinhardtii

莱茵衣藻是研究绿藻产氢的模式物种。为了研究添加外源葡萄糖对莱茵衣藻生长和产氢的影响,本实验通过向莱茵衣藻培养基中加入不同浓度的葡萄糖,检测在光照和黑暗条件下对莱茵衣藻细胞数、叶绿素含量、产氢量和耗氧量的影响,结果表明葡萄糖的添加量为4g.L-1时衣藻的细胞数和叶绿素含量最高,葡萄糖的添加量为6g.L-1时衣藻产氢量比在正常培养基中的提高6.7倍,达156μL.mg-1chl;但在缺硫培养基中添加外源葡萄糖却没有最终使产氢量增加。结果表明向培养基中加入葡萄糖确实提高了产氢量,为利用衣藻进行含糖废水的处理和产氢的研究奠定了实验基础。

Chlamydomonas reinhardtii Dang. is a model species for the study of H2 production because its hydrogenase has a high activity. Glucose can improve the respiration and lead to oxygen deficiency to induce the expression of hydrogenase if it is added into culture medium. In our study, we added different concentrations of glucose into cultures of C.reinhardtii and observed their effects on cell densities, chlorophyll contents, H2 productions and O2 consumptions of C.reinhardtii under light and dark. The highest cell density and chlorophyll content of C.reinhardtii culture was observed when adding 4g·L^-1 glucose in normal culture. The highest H2 production was 156μL·mg-1 chl when adding 6g·L^-1 glucose in normal culture, which was 6.7 times higher than that before adding glucose. However, the H2 production wasn’t improved when adding glucose in the sulfur-deprived culture medium. Our results demonstrated that the H2 production of C.reinhardtii was improved when adding glucose in the normal culture medium, suggesting the potential of using C.reinhardtii in dealing with waster water containing glucose for H2 production.