中草药浸提物对农杆菌抑制和对黑麦草愈伤组织生长、分化影响

Effects of Chinese Herbal Medicines Extracts on Inhibition of Agrobacterium tumefaciens,Growth and Differentiation of Calli from Lolium perenne

采用琼脂块扩散法与滤纸片法检测中草药的抑菌活性,在愈伤组织生长和分化的培养基中加入有抑菌活性的中草药,检验中草药对愈伤组织的影响,筛选可以抑制根癌农杆菌生长,但不影响植物受体组织生长分化的中草药。结果表明,黄芩、蒲公英、连翘和黄连对农杆菌cbf抑制效果明显,黄芩和黄连对农杆菌3301RT有明显的抑制效果;琼脂块扩散法抑菌效果更明显。除黄连之外,黄芩、蒲公英和连翘对黑麦草愈伤组织的生长和分化的影响均不显著。黄芩可以作为良好的抗生素替代品应用于农杆菌介导的植物转化工程。

The antibacterial activity of Chinese herbal medicines was examined by agar-diffusing and filter-paper methods. Chinese herbal medicines were added into development and growth medium to observe the influence on growth and differentiation of calli from Lolium perenne, to select Chinese herbal medicines that possess significant antibacterial activity but do not show negative influence on the growth and differentiation of calli from Lolium perenne. The results showed that Scutellaria baicalensis Georgi, Taraxacum mongolicum, Forsythia suspensa and Coptis chinensis strongly inhibited the effects of A.tumefaciens cbf. A.tumefaciens 3301RT was pronouncedly inhibited by S.baicalensis Georgi and C.chinensis. The antibacterial effects on Agrobacterium tumefaciens by agar-diffusing method were more significant compared to filter-paper method. C.chinensis significantly suppressed the growth and differentiation of calli from L.perenne types. However, the other three Chinese herbal medicines didn’t affect calli’s development. S.baicalensis Georgi could be applied into plant gene engineering mediated by A.tumefaciens.