Cajal样细胞缺失对豚鼠逼尿肌自主收缩的影响及机制探讨

The effect of interstitial cell of Cajal-like cells loss on contraction of guinea-pig detrusor

目的:观察在体内阻断c-kit信号途径造成豚鼠膀胱Cajal样细胞缺失后,豚鼠逼尿肌自主收缩活动的变化,探讨逼尿肌中Cajal样细胞的作用。方法:选取新生豚鼠20只,随机分成实验组、对照组。实验组在出生24h内的豚鼠腹腔内隔天注射c-kit抗体(ACK2)100μg,共5次,对照组腹腔内注射生理盐水,于第10天取膀胱。然后将两组豚鼠膀胱制作冰冻切片行免疫荧光染色及激光共聚焦显微镜观察。制作豚鼠膀胱逼尿肌肌条,记录逼尿肌肌条自主收缩活动的变化。结果:10d后实验组与对照组豚鼠逼尿肌中Cajal样细胞在激光共聚焦显微镜下比较:实验组膀胱逼尿肌中Cajal样细胞数量减少甚至消失(P<0.01)。与对照组比较,实验组逼尿肌肌条收缩幅度、收缩频率明显下降(P<0.01)。结论:Cajal样细胞与豚鼠逼尿肌自发收缩活动密切相关。

Objective：To investigate the effect of interstitial cell of Cajal-like cells loss on contraction of guinea-pig detrusor and the role of Interstitial cell of Cajal-like cells in contraction of guinea-pig detrusor.Methods：New-born guinea-pigs were injected intraperitoneally antibody of c-kit（ACK2）100μg every second day,and control groups were injected saline.The distribution of Cajal-like cells was observed by laser scanning confocal microscope when the guinea-pigs were 10 days postpartum.The strips from detrusor smooth muscle were immersed into Krebs solution and linked with tonotransducer to recode their contraction.Results：Compared with those in the control group,the distribution of interstitial cell of Cajal-like cells was lost,the amplitude and frequency of spontaneous contraction decreased significantly when c-kit was blocked.Conclusion：Interstitial cell of Cajal-like cells may evoke detrusor smooth muscle contraction as pacemakers.