低能离子束对微生物细胞的直接作用和间接作用研究

Study on the Direct and Indirect Action of N + Ion Implantation on D.radiodurans and E.coli

以耐辐射微球菌（Ｄｅｉｎｏｃｏｃｃｕｓｒａｄｉｏｄｕｒａｎｓ）和Ｅ．ｃｏｌｉ为试材，用显微扫描电镜（ＳＥＭ）和电子自旋共振（ＥＳＲ）波谱仪研究了Ｎ＋离子注入对其细胞作用的情况。结果表明，离子注入对生物细胞既存在着直接作用又存在着间接作用；注入离子的动量传递产生了对细胞的直接作用，其能量沉积显示着对细胞的间接作用，而且随着注入剂量的增大，两种微生物细胞受到直接和间接作用损伤的程度也逐渐加剧。

The direct and indirect action of N + ion implantation on D.radiodurans and E.coli was investigated by SEM and ESR spectrum. The results showed that N +ion implantation exerted direct and indirect action on the two kinds of microorganisms and the momentum transfer and energy deposition of implantation ions produced the direct and indirect action on cells, respectively. The level of damage of cells by implantation ion was gradually increased with the increase of the implantation dose.