风味蛋白酶水解菜籽蛋白中2S和12S条件的优化

Optimization of hydrolysis conditions of the 2S and 12S protein from rapeseed by flavourzyme

通过单因素试验和正交试验,研究了风味蛋白酶对菜籽蛋白中2S(RP-2S)和12S(RP-12S)的水解条件。结果表明,水解RP-2S的最佳酶解条件为底物浓度1%,酶与底物浓度比(E/S)95LAPU/g,酶解温度50℃,pH 7.0,酶解时间3 h,此条件下RP-2S的水解度达33.64%;水解RP-12S的最佳酶解条件为底物浓度1%,酶与底物浓度比(E/S)85 LAPU/g,酶解温度50℃,pH 6.6,酶解时间3 h,此条件下RP-12S的水解度达19.97%。

Through the single factor and orthogonal experiment,hydrolysis conditions of 2S and 12S protein from rapeseed by flavourzyme were investigated.The results showed that the optimum hydrolysis conditions of 2S were as follows： substrate concentration 1%,enzyme-substrate ratio（E/S） 95 LAPU/g,temperature 50℃,pH 7.0,hydrolysis time 3 h.Under these conditions the degree of hydrolysis was 33.64%.The optimum hydrolysis conditions of 12S were as follows： substrate concentration 1%,enzyme-substrate ratio（E/S） 85 LAPU/g,temperature 50℃,pH 6.6,hydrolysis time 3 h.Under these conditions the degree of hydrolysis was 19.97%.