内脏脂肪素在糖尿病小鼠心肌中的表达研究

Expression of visfatin in the myocardium of streptozotocin-induced diabetic mouse

目的研究内脏脂肪素在糖尿病小鼠心肌组织中的表达。方法将昆明小鼠随机分为对照(NC)组和糖尿病(DM)组,DM组采用STZ造模。每周检测一次体重、血糖。利用透视电子显微术分析心肌超微结构变化,免疫印迹法观察内脏脂肪素在心肌组织中表达情况。结果 4周后,与NC组相比,DM组体重和血糖显著升高(P<0.05)。DM组心肌细胞超微结构显示染色质凝集、闰盘各带连接明显扩张、线粒体嵴断裂空变、肌丝溶解被大量空泡填充。与NC组相比,DM组心肌组织内脏脂肪素蛋白表达水平增加(P<0.05)。结论高血糖能够破坏心肌细胞,在糖尿病小鼠心肌组织中内脏脂肪素蛋白表达水平增加,可能是一种代偿反应,对心肌起到一定的保护作用。

Objective To study the expression of visfatin in the myocardium of streptozotocin- induced diabetic mice. Methods The kunming mice were randomly divided into the normal and diabetic group. The diabetic model was made by using streptozotocin （STZ）. Weight and level of blood glucose of mouse in diabetic and control groups were measured every week. Ultrastructure changes of heart were analyzed by using transmission electron microscopy. Expression of visfatin in myocardium was explored by Western blot. Results Four weeks after induction of diabetes, the weight, blood glucose were higher in diabetic group than in normal group. Moreover, mouse in diabetic group showed myocardium ultrastructural changes including chromatic agglutination, conjunction of intercalary disc expansion, crista mitochondriales disruption, myofilament solution and vacuolus obturation. A significant increase of visfatin expression was also observed in cardiac myocytes of diabetic mouse （1. 713 ±0. 18 vs 1. 467±0. 12,P〈0. 05）. Conclusions This study provides experimental evidence that hyperglycemia could damage cardiac myocytes. The increase of visfatin expression was also observed in the myocardium of streptozotocin-induced diabetic mice.