中医药治疗寻常型进行期银屑病实验研究

Empirical Research of TCM on Vulgaris Psoriasis in Active Stage

目的:观察风热、血热、湿热不同证型中药复方对实验性银屑病的治疗作用,并观察其对小鼠迟发型超敏反应的作用。方法:利用小鼠尾部表皮天然缺少颗粒层而类似银屑病角质形成细胞分化障碍病理改变,给予小鼠低、中、高3个剂量不同证型中药复方银屑病治疗药物,计数鼠尾颗粒细胞数;选雌性小鼠腹腔注射乙烯雌酚0.2 mg/只,连续3天,3天后改隔日1次至第12天为止,造成小鼠阴道上皮过度增殖模拟银屑病病理改变,分别灌胃给予低、中、高3个剂量风热证、湿热证、血热证中药复方药物,并设阿维A为阳性对照,连续12天,光镜下观察阴道组织病理学变化,计数小鼠阴道基底细胞有丝分裂指数的颗粒总数;以2,4-二硝基氯苯(DNCB)致敏与激敏致小鼠迟发型超敏反应,测定小鼠耳廓肿胀度。结果:中、高剂量风热证方、湿热证方和血热证方的高剂量显著增加小鼠尾部表皮颗粒细胞数;中、高剂量风热证方、湿热证方和血热证方高剂量明显降低雌激素致小鼠阴道基底细胞有丝分裂指数。中、高剂量风热证方和中剂量湿热证方及中剂量血热证方显著降低2,4-二硝基氯苯致小鼠迟发型超敏反应小鼠耳廓肿胀度。结论:风热证方、湿热证方、血热证方均可改善小鼠尾部表皮类似银屑病角质形成细胞分化障碍,抑制小鼠迟发性过敏反应。临床对风热、血热、湿热不同证型银屑病疗效可靠。

Objective： To observe the curative effects of complex prescriptions of such different syndromes as wind-heat syndrome,blood-heat syndrome and humid-heat syndrome on experimental psoriasis,and the effect on delayed hypersensitivity reaction in mice.Method： To draw assistance from mouse tail＇s lack of granular layer similar to psoriasis in which malpighian cell has dysdifferentiation,we gave mice complex prescriptions for different types of syndromes in low,medium,and high doses to do treatment.Then we counted the numbers of granular cells in mice tails.Then the female mice were chosen to inject Diethylstilbestrol by intraperitoneal injection,0.2mg each mouse.After 3 days the frequency was changed into once every other day for 12 days,and vagina endepidermis was caused to hypernomic hyperplasy similar to pathology of psoriasis.So complex prescriptions were given to them for Wind-heat Syndrome,Blood-heat Syndrome,humid-heat syndrome in low,medium,and high doses by intragastric administration,and Acitretin was set up to be positive control drug.For 12 days histopathological change in vagina was observed under light microscope and mitotic index of basal cell was counted in mice vagina.2,4-dinitro-chlorobenzene sensitized the mice to cause delayed hypersensitivity,and the grade of pinna swelling was detected.Result： Prescription for wind-heat syndrome and prescription for damp-heat syndrome in low and high doses and prescription for blood-heat syndrome in high dose could markedly increase granular cells in mice tail＇s epidermis,and could cut down mitotic index of basal cell in mice vagina.Prescription for wind-heat syndrome in medium and high doses,prescription for damp-heat syndrome in medium dose and prescription for blood-heat syndrome in medium dose could degrade the grade of mice pinna swelling caused by delayed hypersensitivity with 2,4-dinitro-chlorobenzene.Conclusion： Prescription for Wind-heat Syndrome,Prescription for Blood-heat Syndrome and Prescription for Damp-heat syndrome can improve the dysdifferentiation of malpighian cells similar to psoriasis in cuticulum of mouse＇s end part,and inhibit mouse＇s delayed hypersensitivity reaction.They show reliable curative effects on psoriasis in various syndromes.