发酵产薯蓣皂苷酶的反应条件

Production and properties of dioscin-glycosidase

研究了酶法水解黄姜薯蓣皂苷分子上的糖基,使皂苷水解生成无糖基、高活性皂苷元的条件。探讨了4种菌株对薯蓣皂苷糖基的水解能力,筛选出1种薯蓣皂苷酶高产菌株Absidia sp.s00,用于水解薯蓣皂苷。通过薄层层析法分析得到薯蓣皂苷酶解最佳反应条件为40℃p、H 5.0,反应30 h。在此最佳条件下进行酶反应,1 g黄姜薯蓣皂苷底物水解后,酶解产物经分离纯化,得薯蓣皂苷元0.1 g,采用高效液相色谱检测其纯度为90%以上。

Absidia sp.s00 was used to produced dioscin-glycosidase from four tested species.The optimal conditions for enzymatic hydrolysis of dioscin were pH 5.0 at 40 ℃ for 30 h.According to optimal conditions,1 g dioscin hydrolyzed by dioscin-glycosidase can obtain 0.1 g diosgenin.The purity of diosgenin was over 90% by HPLC.