高效液相色谱-串联质谱法测定人参中的多菌灵残留

Determination of carbendazim in ginseng by liquid chromatography-tandem mass spectrometry

在酸性条件下以甲醇提取人参样品中的多菌灵残留,用高效液相色谱-串联质谱法进行检测。以体积分数0.1%的甲酸-甲醇水溶液梯度洗脱,样品的提取方法为固液萃取,流动相为甲醇,体积流量0.2 mL/min,运行时间10 min。质谱采用正离子扫描模式,定性碎片离子是192.10/160.05、192.10/132.06、192.10/105.06,定量碎片离子为192.10/160.05。仪器检出限和方法检出限分别是0.5 pg和0.022 ng/g,相对标准偏差为1.32%～2.29%,回收率为88.23%～95.07%。研究表明该方法的精准度以及灵敏度均达到要求,适用于人参中多菌灵残留的检测。

Samples were extracted by liquid-solid extraction method,then determined by HPLC-Ms/Ms.The separation of carbendazim was performed,using gradient elution of methanol-water（containing 0.1% methane acid）.The mobile phase was acetonitrile,while the flow rate and running time were 0.2 mL/min and 10 min.Positive ions were used for the scan mode of mass spectrometry,fragment ions used for quantitative analysis were 192.10/160.05,192.10/132.06 and 192.10/105.06,for qualitative was 192.10/160.05.Detection limits of the compound and method detection limit were 0.5 pg and 0.022 ng/g respectively.The recoveries of carbendazim were 88.23% to 95.07%,and the relative standard deviation of this method was between 1.3% to 2.2%.