摘要
目的研究肠化胃粘膜幽门螺杆菌(Hp)阳性率与PCNA,cerbB2表达率之间关系,以探讨Hp感染在胃肠化发生、发展中作用.方法经病理检查证实的慢性胃炎伴肠化116例,对照组非溃疡性消化不良.应用改良WarthinStary法检测Hp,免疫酶组化SP法检测PCNA,cerbB2的表达,比较Hp阳性组和阴性组间PCNA,cerbB2的阳性表达率.结果胃粘膜肠化者Hp感染率增高(586%vs188%,χ2=1079,P<001),肠化胃上皮内少见Hp粘附,Hp阳性组PCNA,cerbB2表达高于阴性组(48/68vs12/48,χ2=905,P<005;36/74vs2/42,χ2=1328,P<001).结论Hp感染促进胃粘膜肠化,并使肠化胃粘膜细胞增殖迅速而启动恶性变,故Hp感染可能促进胃癌的形成.
AIM To study the prevalence of Helicobacter pylori (Hp) and the relation between expression of PCNA, c erbB 2 and Hp infection in gastric mucosa with intestinal metaplasia to evaluate the role of Hp infection in morphogenesis and development of intestinal metaplasia. METHODS Pathologically confirmed 116 cases of chronic gastritis with intestinal metaplasia and 32 controls of non ulcer dyspepsia were studied. Hp was detected by using improved Warthin Starry method and PCNA, c erbB 2 were examined by using immunohistochemical method (SP system). The expressions of PCNA and c erbB 2 were studied in Hp positive and Hp negative patients. RESULTS The prevalence of Hp increased significantly in gastric mucosa with intestinal metaplasia (58 6% vs 18 8%, χ 2=10 79, P <0 01), but Hp rarely colonized in gastric epithelium with intestinal metaplasia. The expressions of PCNA and c erbB 2 in Hp positive patients was significantly higher than those in Hp negative patients (48/68 vs 12/48 , χ 2=9 05, P <0 05; 36/74 vs 2/42 , χ 2=13 28, P <0 01). CONCLUSION Hp plays a promotional role in morphogenesis of intestinal metaplasia, and stimulates cell over proliferation and gastric epithelium malignant change. So Hp infection may play an important role in gastric carcinogenesis.
出处
《世界华人消化杂志》
CAS
1999年第2期111-113,共3页
World Chinese Journal of Digestology
基金
福建省科委基金
关键词
胃粘膜
肠化生
C-ERBB-2
幽门螺杆菌
胃癌
Helicobacter pylori infection
gastric mucosa
intestinal metaplasia
proliferating cell nuclear antigen
c erbB 2 gene
gastritis
gene expression