木通所致大鼠急性肾损伤的实验观察

EXPERIMENTAL STUDY OF ACUTE NEPHROTOXICITY INDUCED BY ARISTOLOCHIA MANSHURIENSIS KOM IN RATS

目的：观察木通所致大鼠急性肾损伤作用的功能变化及组织形态学特点。 方法：用木通水煎剂（６０ｇ／ｋｇ·ｄ －１） 给大鼠灌胃，建立大鼠急性肾损伤动物模型，检测有关肾功能指标，进行组织形态学观察。 结果：木通组大鼠的主要功能改变为：氮质血症、低渗尿、蛋白尿、糖尿、尿ＮＡＧ 酶升高。组织形态学改变主要表现为：急性肾小管坏死，以皮、髓交界区小管损伤为主。 结论：大剂量木通可导致大鼠急性肾功能衰竭。

To investigate the clinical features and pathological changes of acute nephrotoxicity induced by Aristolochia Manshuriensis Kom (AMK,a Chinese herb)in rats. METHODOLOGY Experimental model of acute renal injury was established in the Sprague Dawley rats with oral administration of decoction of AMK in a dosage of 60g/kg body weight for 5 consecutive days.Serum urea and creatinine levels,urinary activity of N acetyl beta D glucosaminidase(NAG),urinary protein,glucose,osmolality and volume were assayed on the day 0,2,4,6,8 of the experiment after acute renal injury.Renal histological examination was also performed. RESULTS The important renal functional changes of nephrotoxicity due to AMK included azotemia,oliguria,proteinuria,glycouria,urinary hypoosmolality,and NAG enzymuria.Histopathological changes in the kidneys of the treated animals showed evidences of acute tubular necrosis,predominantly in the area of the outer stripe of outer medulla. CONCLUSION Acute renal tubular necrosis occurs in the Sprague Dawley rats after oral administration of AMK in a dosage of 60g/kg body weight for 5 consecutive days.This Chinese herb is thought to be nephrotoxic and should be carefully used in the medical practice.