慢性吗啡处理及戒断后大鼠海马CA1区钙结合蛋白Calbindin的表达变化

Expression of Calbindin in CA1 Area of Hippocam Pus in Rats after Chronic Morphine Treatment and Withdrawal

目的:观察慢性吗啡处理及戒断后大鼠海马CA1区中钙结合蛋白Calbindin(CB)的表达变化,为其功能研究提供形态学依据。方法:36只雄性SD大鼠随机分为吗啡依赖组和生理盐水对照组。吗啡依赖组大鼠腹膜腔注射吗啡,2次/d,起始剂量为5mg/kg,逐日递增5mg/kg,至第10d为50mg/kg;对照组注射同体积的生理盐水。于末次注射后动物分别存活3h、3d、14d和30d。免疫组化方法和相对平均灰度值检测海马CA1区CB的表达变化。结果:在生理盐水处理组各存活时间点海马CA1区CB的表达相同。和生理盐水对照组相比,吗啡依赖组3h时海马CA1区CB的表达明显增加(P<0.05),3d时进一步增加,至第14d时CA1区CB的表达开始恢复。结论:慢性吗啡处理及戒断后海马CA1区CB的表达上调可能对戒断早期海马神经元有保护作用。

Objective：To investigate the expression of calbindin（CB） in the rat CA1 area of hippocampus after chronic morphine treatment and withdrawal,and provide the morphologic evidence for its functional research.Method：Thirty-six SD male rats were randomly divided into morphine treated and saline control groups.The rats in morphine treated group were intraperitoneally injected with morphine twice a day for 10 days.The initial dose of injected morphine was 5 mg/kg,then the dose was added as 5 mg/kg day by day.To 10th day,the dose reached to the 50 mg/kg.The rats in saline treated group were injected with same volume of saline.Rats in both group swere killed 3 hours,3 days or 14 days after the last injection.The CB expression in the rat CA1 area of hippocampus was detected by immunohistochemistry and the mean of relative gray scale.Result：The results showed that the CB expression in the rat CA1 area of hippocampus was same among the survival time points in the saline treated group.Compared to the saline treated group,the CB expression in CA1 area of hippocampus was significantly increased at 3 h,3d after the last injection of morphine（P0.05）,then restored at 14d.Conclusion：The increase expression of CB in CA1 area of hippocampus may have a protective role after chronic morphine treatment and withdrawal.